PUBLICATION

Aquaporin 8ab is required in zebrafish embryonic intestine development

Authors

Wang, S., Qin, Y., Sheng, J., Duan, X., Shen, L., Liu, D.

ID

ZDB-PUB-220727-7

Date

2022

Source

Acta biochimica et biophysica Sinica 54: 1-9 (Journal)

Registered Authors

Liu, Dong, Qin, Yinyin

Keywords

aqp8ab, gastrointestinal tract, intestinal lumen, intestine development, zebrafish

MeSH Terms

Animals
Aquaporins*/genetics
Aquaporins*/metabolism
Intestinal Mucosa/metabolism
Intestines
Mammals/genetics
Mammals/metabolism
RNA, Messenger/genetics
Zebrafish*/genetics
Zebrafish*/metabolism

(all 10)

PubMed

35880566 Full text @ Acta. Biochim. Biophys. Sin (Shanghai)

Abstract

The aquaporin 8 (AQP8) is a small integral membrane protein that selectively transports water and other small uncharged solutes across cell plasma membranes. It has been demonstrated that AQP8 is ubiquitously present in various tissues and organs of mammals, and participates in many physiological and pathological processes. Recent studies showed that AQP8 is highly expressed in the columnar epithelial cells of mammalian colonic mucosa facing lumen, indicating that AQP8 plays potential roles in the physiology and pathophysiology of gastrointestinal tract. However, the role of AQP8 during gastrointestinal tract development is unclear. In the present study, RT-PCR results reveal that the zebrafish genome encodes three kinds of aqp8s ( aqp8aa, aqp8ab, and aqp8b). We use whole mount in situ hybridization to describe aqp8 genes spatiotemporal expression pattern, and the results show that aqp8ab mRNA is detectable mainly in the zebrafish embryonic intestine. To reveal the details of aqp8ab distribution, histological sections are employed. Transverse sections indicate that aqp8ab mRNA expression is more intense in the layer lining the intestinal cavity. Knockout of aqp8ab using the CRISPR/Cas9 system induces intestine development defects and abnormal formation of intestinal lumen. In addition, aqp8ab mRNA significantly rescues the intestine defects in the aqp8ab mutant. These results indicate that aqp8ab is required in the intestine development of zebrafish.

Genes / Markers

Figures

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Expression

Phenotype

Fish	Conditions	Stage	Phenotype	Figure
aqp8a.2^{zf3698/zf3698} (AB)	standard conditions	Protruding-mouth	intestine morphology, normal	Fig. 6
aqp8a.2^{zf3698/zf3698} (AB)	standard conditions	Protruding-mouth	intestine morphology, normal	Fig. 4
aqp8a.2^{zf3698/zf3698} (AB)	standard conditions	Protruding-mouth	pericardium edematous, abnormal	Fig. 4
aqp8a.2^{zf3698/zf3698} (AB)	standard conditions	Protruding-mouth	whole organism decreased length, abnormal	Fig. 4
aqp8a.2^{zf3698/zf3698} (AB)	standard conditions	Day 4	intestine fabp2 expression spatial pattern, abnormal	Fig. 6
aqp8a.2^{zf3698/zf3698} (AB)	standard conditions	Day 4	intestine fabp2 expression spatial pattern, abnormal	Fig. 5
aqp8a.2^{zf3698/zf3698} (AB)	standard conditions	Day 4	intestine fabp2 expression spatial pattern, abnormal	Fig. 4
aqp8a.2^{zf3698/zf3698} (AB)	standard conditions	Day 4	intestine bifurcated, abnormal	Fig. 6
aqp8a.2^{zf3698/zf3698} (AB)	standard conditions	Day 4	intestine bifurcated, abnormal	Fig. 5
aqp8a.2^{zf3698/zf3698} (AB)	standard conditions	Day 4	intestine bifurcated, abnormal	Fig. 4

1 - 10 of 117

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Mutations / Transgenics

Allele	Type	Affected Genomic Region
zf3698	Small Deletion	aqp8a.2
zf3699	Small Deletion	aqp8a.2
zf3700	Small Deletion	aqp8a.2

Human Disease / Model

Sequence Targeting Reagents

Target	Reagent	Reagent Type
aqp8a.2	CRISPR1-aqp8a.2	CRISPR

Fish

Fish
AB
aqp8a.2^{zf3698/zf3698} (AB)
aqp8a.2^{zf3699/zf3699} (AB)
aqp8a.2^{zf3700/zf3700} (AB)

Antibodies

Orthology

Engineered Foreign Genes

Mapping

Wang et al., 2022 ZDB-PUB-220727-7 PMID:35880566

Aquaporin 8ab is required in zebrafish embryonic intestine development

Wang et al., 2022

ZDB-PUB-220727-7
PMID:35880566