PUBLICATION
inka1b expression in the head mesoderm is dispensable for facial cartilage development
- Authors
- Jeon, H., Jin, S., Choe, C.P.
- ID
- ZDB-PUB-220712-4
- Date
- 2022
- Source
- Gene expression patterns : GEP 45: 119262 (Journal)
- Registered Authors
- Keywords
- Facial cartilages, Head mesoderm, Zebrafish, inka1b
- MeSH Terms
-
- Animals
- Cartilage/metabolism
- Gene Expression Regulation, Developmental
- Mesoderm/metabolism
- Mice
- Neural Crest*/metabolism
- Zebrafish*/genetics
- Zebrafish*/metabolism
- Zebrafish Proteins/genetics
- Zebrafish Proteins/metabolism
- PubMed
- 35811016 Full text @ Gene Expr. Patterns
Citation
Jeon, H., Jin, S., Choe, C.P. (2022) inka1b expression in the head mesoderm is dispensable for facial cartilage development. Gene expression patterns : GEP. 45:119262.
Abstract
Inka box actin regulator 1 (Inka1) is a novel protein identified in Xenopus and is found in vertebrates. While Inka1 is required for facial skeletal development in Xenopus and zebrafish, it is dispensable in mice despite its conserved expression in the cranial neural crest, indicating that Inka1 function in facial skeletal development is not conserved among vertebrates. Zebrafish bears two paralogs of inka1 (inka1a and inka1b) in the genome, with the biological roles of inka1b barely known. Here, we analyzed the expression and function of inka1b during facial skeletal development in zebrafish. inka1b was expressed sequentially in the head mesoderm adjacent to the pharyngeal pouches essential for facial skeletal development at the stage of arch segmentation. However, a loss-of-function mutation in inka1b displayed normal head development, including the pouches and facial cartilages. The normal head of inka1b mutant fish was unlikely a result of the genetic redundancy of inka1b with inka1a, given the distinct expression of inka1a and inka1b in the cranial neural crest and head mesoderm, respectively, during craniofacial development. Our findings suggest that the inka1b expression in the head mesoderm might not be essential for head development in zebrafish.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping