PUBLICATION
A NIR fluorescent probe for the specific detection of hypochlorite and its application in vitro and in vivo
- Authors
- Zhen, L., Lan, J., Zhang, S., Liu, L., Zeng, R., Chen, Y., Ding, Y.
- ID
- ZDB-PUB-220526-2
- Date
- 2022
- Source
- Analytical methods : advancing methods and applications 14(22): 2147-2152 (Journal)
- Registered Authors
- Chen, Yi
- Keywords
- none
- MeSH Terms
-
- Animals
- Fluorescent Dyes*
- Hypochlorous Acid*
- Limit of Detection
- Microscopy, Fluorescence/methods
- Zebrafish
- PubMed
- 35611957 Full text @ Anal Methods
Citation
Zhen, L., Lan, J., Zhang, S., Liu, L., Zeng, R., Chen, Y., Ding, Y. (2022) A NIR fluorescent probe for the specific detection of hypochlorite and its application in vitro and in vivo. Analytical methods : advancing methods and applications. 14(22):2147-2152.
Abstract
It is of great necessity to exploit a real-time, highly selective and sensitive method for hypochlorite (ClO-) detection in both the environment and living systems because of the complex influence of ClO- on health. In this paper, based on the intramolecular charge transfer (ICT) effect, a NIR fluorescent probe (probe DAB) was designed for the accurate detection of ClO-, which produced a fluorescence response to ClO- with high selectivity and rapid response (within 1 min). The probe DAB could determine ClO- over the linear range of 0-80 μM with a low detection limit of 1.46 μM. And the sensing mechanism between the probe and ClO- was verified using HPLC and MS. To further prove its practicability, the probe was applied for detecting ClO- in actual water samples. In addition, owing to its good sensing properties and low cytotoxicity, probe DAB could be expediently applied to visualize ClO- in living cells and zebrafish, and it is expected to be a useful tool for investigating the detailed functions and mechanisms of ClO- in living systems.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping