PUBLICATION

Zinc pyrithione (ZPT) -induced embryonic toxicogenomic responses reveal involvement of oxidative damage, apoptosis, endoplasmic reticulum (ER) stress and autophagy

Authors
Zhao, Y., Wang, H., Duah, P.A., Retyunskiy, V., Liu, Y., Chen, G.
ID
ZDB-PUB-220521-9
Date
2022
Source
Aquatic toxicology (Amsterdam, Netherlands)   248: 106195 (Journal)
Registered Authors
Keywords
ER stress, Zinc pyrithione, apoptosis, autophagy, oxidative stress
MeSH Terms
  • Animals
  • Apoptosis
  • Autophagy
  • Endoplasmic Reticulum
  • Endoplasmic Reticulum Stress
  • Organometallic Compounds
  • Oxidative Stress
  • Pyridines
  • Toxicogenetics
  • Water Pollutants, Chemical*/toxicity
  • Zebrafish*/genetics
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PubMed
35594629 Full text @ Aquat. Toxicol.
Abstract
Zinc pyrithione (ZPT) is a frequently used organometallic biocide, carrying potentially adverse consequences to multiple species in the environment. Previously we have demonstrated its embryonic, organ developmental and liver metabolic toxicity of zebrafish. However, details of ZPT toxicity during embryogenesis are still limited. The present study was designed to evaluate the effects and possible mechanisms of ZPT-induced embryonic toxicogenomic responses by morphological investigations, transcriptome and gene quantitative analysis, as well as biochemical assays. The results revealed that treatment with ZPT caused embryogenesis toxicity, specifically in irregular cell division and rearrangement, delayed differentiations of eyes and notochords, the epiboly and germ ring formation and somite segmentation defects. In addition, ZPT exposure altered gene expression during early embryonic development, especially related with morphological abnormities and metabolic dysfunctions including reduction of oxidoreductase activity. Activities of antioxidants and caspases examinations showed inductions of oxidative stress and apoptosis by ZPT and quantitative analysis of marker genes further indicated that ZPT also triggered endoplasmic reticulum (ER) stress and autophagy. Thus, we deduce here that ZPT-induced embryonic toxicogenomic responses reveal involvement of oxidative damage, apoptosis, endoplasmic reticulum (ER) stress and autophagy.
Genes / Markers
Marker Marker Type Name
actb2GENEactin, beta 2
apaf1GENEapoptotic peptidase activating factor 1
atg5GENEATG5 autophagy related 5 homolog (S. cerevisiae)
baxaGENEBCL2 associated X, apoptosis regulator a
bcl2aGENEBCL2 apoptosis regulator a
becn1GENEbeclin 1, autophagy related
casp3aGENEcaspase 3, apoptosis-related cysteine peptidase a
casp9GENEcaspase 9, apoptosis-related cysteine peptidase
caspbGENEcaspase b
catGENEcatalase
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Figures
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Expression
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay Figure
apaf1TUchemical treatment by environment: zinc pyrithionePrim-5RTPCR
atg5TUchemical treatment by environment: zinc pyrithionePrim-5RTPCR
baxaTUchemical treatment by environment: zinc pyrithionePrim-5RTPCR
bcl2aTUchemical treatment by environment: zinc pyrithionePrim-5RTPCR
bcl2aTUchemical treatment by environment: zinc pyrithionePrim-5RNA-Seq
bcl2aTUchemical treatment by environment: zinc pyrithionePrim-5RTPCR
casp3aTUchemical treatment by environment: zinc pyrithionePrim-5RTPCR
casp9TUchemical treatment by environment: zinc pyrithionePrim-5RTPCR
caspbTUchemical treatment by environment: zinc pyrithionePrim-5RTPCR
caspbTUchemical treatment by environment: zinc pyrithionePrim-5RNA-Seq
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Phenotype
Fish Conditions Stage Phenotype Figure
TUchemical treatment by environment: zinc pyrithione2-cell to 512-cell
TUchemical treatment by environment: zinc pyrithioneGerm-ring to Shield
TUchemical treatment by environment: zinc pyrithioneGerm-ring to 75%-epiboly
TUchemical treatment by environment: zinc pyrithione5-9 somites
TUchemical treatment by environment: zinc pyrithione5-9 somites
TUchemical treatment by environment: zinc pyrithione5-9 somites
TUchemical treatment by environment: zinc pyrithione20-25 somites
TUchemical treatment by environment: zinc pyrithione20-25 somites
TUchemical treatment by environment: zinc pyrithione20-25 somites
TUchemical treatment by environment: zinc pyrithione20-25 somites
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Mutations / Transgenics
No data available
Human Disease / Model
No data available
Sequence Targeting Reagents
No data available
Fish
Fish
TU
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Antibodies
Orthology
Engineered Foreign Genes
No data available
Mapping
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Citation
Zhao, Y., Wang, H., Duah, P.A., Retyunskiy, V., Liu, Y., Chen, G. (2022) Zinc pyrithione (ZPT) -induced embryonic toxicogenomic responses reveal involvement of oxidative damage, apoptosis, endoplasmic reticulum (ER) stress and autophagy. Aquatic toxicology (Amsterdam, Netherlands). 248:106195.