PUBLICATION

GATA4/5/6 family transcription factors are conserved determinants of cardiac versus pharyngeal mesoderm fate

Authors
Song, M., Yuan, X., Racioppi, C., Leslie, M., Stutt, N., Aleksandrova, A., Christiaen, L., Wilson, M.D., Scott, I.C.
ID
ZDB-PUB-220312-5
Date
2022
Source
Science advances   8: eabg0834 (Journal)
Registered Authors
Aleksandrova, Anastasiia, Leslie, Meaghan, Scott, Ian, Song, Mengyi, Yuan, Xuefei
Keywords
none
MeSH Terms
  • Animals
  • GATA4 Transcription Factor*/genetics
  • GATA4 Transcription Factor*/metabolism
  • GATA5 Transcription Factor/genetics
  • GATA5 Transcription Factor/metabolism
  • Gene Expression Regulation, Developmental
  • Mesoderm/metabolism
  • Zebrafish*/genetics
  • Zebrafish*/metabolism
(all 9)
PubMed
35275720 Full text @ Sci Adv
Abstract
GATA4/5/6 transcription factors play essential, conserved roles in heart development. To understand how GATA4/5/6 modulates the mesoderm-to-cardiac fate transition, we labeled, isolated, and performed single-cell gene expression analysis on cells that express gata5 at precardiac time points spanning zebrafish gastrulation to somitogenesis. We found that most mesendoderm-derived lineages had dynamic gata5/6 expression. In the absence of Gata5/6, the population structure of mesendoderm-derived cells was substantially altered. In addition to the expected absence of cardiac mesoderm, we confirmed a concomitant expansion of cranial-pharyngeal mesoderm. Moreover, Gata5/6 loss led to extensive changes in chromatin accessibility near cardiac and pharyngeal genes. Functional analyses in zebrafish and the tunicate Ciona, which has a single GATA4/5/6 homolog, revealed that GATA4/5/6 acts upstream of tbx1 to exert essential and cell-autonomous roles in promoting cardiac and inhibiting pharyngeal mesoderm identity. Overall, cardiac and pharyngeal mesoderm fate choices are achieved through an evolutionarily conserved GATA4/5/6 regulatory network.
Genes / Markers
Figures
Figure Gallery (9 images)
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Expression
Phenotype
No data available
Mutations / Transgenics
Allele Construct Type Affected Genomic Region
b109
    		Small Deletion
    fb7TgTransgenic Insertion
      hsc115
        		Small Deletion
        hsc116
          		Small Deletion
          hsc171TgTransgenic Insertion
            hsc172TgTransgenic Insertion
              hsc173TgTransgenic Insertion
                hsc174TgTransgenic Insertion
                  hsc175TgTransgenic Insertion
                    hsc176TgTransgenic Insertion
                      1 - 10 of 27
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                      Human Disease / Model
                      No data available
                      Sequence Targeting Reagents
                      Target Reagent Reagent Type
                      gata5CRISPR3-gata5CRISPR
                      gata5MO1-gata5MRPHLNO
                      gata6CRISPR1-gata6CRISPR
                      gata6MO1-gata6MRPHLNO
                      1 - 4 of 4
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                      Fish
                      No data available
                      Antibodies
                      No data available
                      Orthology
                      No data available
                      Engineered Foreign Genes
                      Marker Marker Type Name
                      DsRedEFGDsRed
                      DsRed2EFGDsRed2
                      EGFPEFGEGFP
                      GFPEFGGFP
                      mRFPEFGmRFP
                      ZsYellowEFGZsYellow
                      1 - 6 of 6
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                      Mapping
                      No data available
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                      Citation
                      Song, M., Yuan, X., Racioppi, C., Leslie, M., Stutt, N., Aleksandrova, A., Christiaen, L., Wilson, M.D., Scott, I.C. (2022) GATA4/5/6 family transcription factors are conserved determinants of cardiac versus pharyngeal mesoderm fate. Science advances. 8:eabg0834.