PUBLICATION

Single-cell RNA analysis identifies pre-migratory neural crest cells expressing markers of differentiated derivatives

Authors
Lencer, E., Prekeris, R., Artinger, K.B.
ID
ZDB-PUB-210817-10
Date
2021
Source
eLIFE   10: (Journal)
Registered Authors
Artinger, Kristin Bruk
Keywords
developmental biology, neural crest cells, pigment development, rohon-beard cells, scRNA-seq, xanthophores, zebrafish
Datasets
GEO:GSE163907
MeSH Terms
  • Animals
  • Cell Lineage
  • Cell Movement*
  • Embryo, Nonmammalian
  • Gene Expression
  • Genetic Markers*
  • Neural Crest/physiology*
  • Neurons/physiology
  • Sequence Analysis, RNA*
  • Single-Cell Analysis*
  • Zebrafish/embryology*
(all 11)
PubMed
34397384 Full text @ Elife
Abstract
The neural crest is a migratory population of stem-like cells that contribute to multiple traits including the bones of the skull, peripheral nervous system, and pigment. How neural crest cells differentiate into diverse cell types is a fundamental question in the study of vertebrate biology. Here, we use single-cell RNA sequencing to characterize transcriptional changes associated with neural crest cell development in the zebrafish trunk during the early stages of migration. We show that neural crest cells are transcriptionally diverse and identify pre-migratory populations already expressing genes associated with differentiated derivatives, specifically in the xanthophore lineage. Further, we identify a population of Rohon-Beard neurons in the data. The data presented identify novel genetic markers for multiple trunk neural crest cell populations and Rohon-Beard neurons providing insight into previously uncharacterized genes critical for vertebrate development.
Genes / Markers
Marker Marker Type Name
aox5GENEaldehyde oxidase 5
aqp3aGENEaquaporin 3a
ccdc39GENEcoiled-coil domain 39 molecular ruler complex subunit
cnmdGENEchondromodulin
col2a1bGENEcollagen, type II, alpha 1b
crestinGENEcrestin
cxcr4bGENEchemokine (C-X-C motif), receptor 4b
dlx2aGENEdistal-less homeobox 2a
drgxGENEdorsal root ganglia homeobox
elavl3GENEELAV like neuron-specific RNA binding protein 3
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Figures
Figure Gallery (4 images)
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Expression
Phenotype
Fish Conditions Stage Phenotype Figure
prdm1am805/m805standard conditionsPrim-5
prdm1am805/m805standard conditionsPrim-5
prdm1am805/m805standard conditionsPrim-5
prdm1am805/m805standard conditionsPrim-5
prdm1am805/m805standard conditionsPrim-5
1 - 5 of 5
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Mutations / Transgenics
Allele Construct Type Affected Genomic Region
ba2TgTransgenic Insertion
    co26TgTransgenic Insertion
      m805
        		Point Mutation
        1 - 3 of 3
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        Human Disease / Model
        No data available
        Sequence Targeting Reagents
        Fish
        Fish
        ba2Tg
        co26Tg
        prdm1am805/m805
        WT
        1 - 4 of 4
        Show
        Antibodies
        Orthology
        Engineered Foreign Genes
        Marker Marker Type Name
        EGFPEFGEGFP
        TagRFPEFGTagRFP
        1 - 2 of 2
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        Mapping
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        Citation
        Lencer, E., Prekeris, R., Artinger, K.B. (2021) Single-cell RNA analysis identifies pre-migratory neural crest cells expressing markers of differentiated derivatives. eLIFE. 10:.