PUBLICATION
Upregulation of GBP1 in thyroid primordium is required for developmental thyroid morphogenesis
- Authors
- Yang, R.M., Zhan, M., Zhou, Q.Y., Ye, X.P., Wu, F.Y., Dong, M., Sun, F., Fang, Y., Zhang, R.J., Zhang, C.R., Yang, L., Guo, M.M., Zhang, J.X., Liang, J., Cheng, F., Liu, W., Han, B., Zhou, Y., Zhao, S.X., Song, H.D.
- ID
- ZDB-PUB-210702-7
- Date
- 2021
- Source
- Genetics in medicine : official journal of the American College of Medical Genetics 23(10): 1944-1951 (Journal)
- Registered Authors
- Dong, Mei, Song, Huai-Dong, Wu, Fengyao, Yang, Ruimeng, Zhou, Yi
- Keywords
- none
- MeSH Terms
-
- Animals
- Congenital Hypothyroidism*/genetics
- Disease Models, Animal
- GTP-Binding Proteins*/genetics
- Humans
- Morphogenesis
- Mutation
- Thyroid Dysgenesis*
- Thyroid Gland/growth & development*
- Up-Regulation
- Zebrafish/genetics
- PubMed
- 34194003 Full text @ Genet. Med.
Citation
Yang, R.M., Zhan, M., Zhou, Q.Y., Ye, X.P., Wu, F.Y., Dong, M., Sun, F., Fang, Y., Zhang, R.J., Zhang, C.R., Yang, L., Guo, M.M., Zhang, J.X., Liang, J., Cheng, F., Liu, W., Han, B., Zhou, Y., Zhao, S.X., Song, H.D. (2021) Upregulation of GBP1 in thyroid primordium is required for developmental thyroid morphogenesis. Genetics in medicine : official journal of the American College of Medical Genetics. 23(10):1944-1951.
Abstract
Purpose Congenital hypothyroidism (CH) is a common congenital endocrine disorder in humans. CH-related diseases such as athyreosis, thyroid ectopy, and hypoplasia are primarily caused by dysgenic thyroid development. However, the underlying molecular mechanisms remain unknown.
Methods To identify novel CH candidate genes, 192 CH patients were enrolled, and target sequencing of 21 known CH-related genes was performed. The remaining 98 CH patients carrying no known genes were subjected to exome sequencing (ES). The functions of the identified variants were confirmed using thyroid epithelial cells in vitro and in zebrafish model organisms in vivo.
Results Four pathogenic GBP1 variations from three patients were identified. In zebrafish embryos, gbp1 knockdown caused defective thyroid primordium morphogenesis and hypothyroidism. The thyroid cells were stuck together and failed to dissociate from each other to form individual follicles in gbp1-deficient embryos. Furthermore, defects were restored with wild-type human GBP1 (hGBP1) messenger RNA (mRNA) except for mutated hGBP1 (p.H150Y, p.L187P) overexpression. GBP1 promoted β-catenin translocation into the cytosol and suppressed the formation of cellular adhesion complexes. Suppression of cell-cell adhesion restored the thyroid primordium growth defect observed in gbp1-deficient zebrafish embryos.
Conclusion This study provides further understanding regarding thyroid development and shows that defective cellular remodeling could cause congenital hypothyroidism.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping