PUBLICATION

The Lysine Methylase SMYD3 Modulates Mesendodermal Commitment during Development

Authors

Fittipaldi, R., Floris, P., Proserpio, V., Cotelli, F., Beltrame, M., Caretti, G.

ID

ZDB-PUB-210603-22

Date

2021

Source

Cells 10(5): (Journal)

Registered Authors

Beltrame, Monica, Cotelli, Franco

Keywords

SMYD3, development, embryonic stem cells, zebrafish

MeSH Terms

Animals
Cell Differentiation*
Cell Line
Cell Lineage
Embryonic Development
Gene Expression Regulation, Developmental
Histone-Lysine N-Methyltransferase/genetics
Histone-Lysine N-Methyltransferase/metabolism*
Mice
Mouse Embryonic Stem Cells/enzymology*
Time Factors
Zebrafish/embryology
Zebrafish/genetics
Zebrafish/metabolism*
Zebrafish Proteins/genetics
Zebrafish Proteins/metabolism*

(all 16)

PubMed

34069776 Full text @ Cells

Abstract

SMYD3 (SET and MYND domain containing protein 3) is a methylase over-expressed in cancer cells and involved in oncogenesis. While several studies uncovered key functions for SMYD3 in cancer models, the SMYD3 role in physiological conditions has not been fully elucidated yet. Here, we dissect the role of SMYD3 at early stages of development, employing mouse embryonic stem cells (ESCs) and zebrafish as model systems. We report that SMYD3 depletion promotes the induction of the mesodermal pattern during in vitro differentiation of ESCs and is linked to an upregulation of cardiovascular lineage markers at later stages. In vivo, smyd3 knockdown in zebrafish favors the upregulation of mesendodermal markers during zebrafish gastrulation. Overall, our study reveals that SMYD3 modulates levels of mesendodermal markers, both in development and in embryonic stem cell differentiation.

Genes / Markers

Marker	Marker Type	Name
chrd	GENE	chordin
dmbx1a	GENE	diencephalon/mesencephalon homeobox 1a
gata2a	GENE	GATA binding protein 2a
gata5	GENE	GATA binding protein 5
gata6	GENE	GATA binding protein 6
gsc	GENE	goosecoid
kdrl	GENE	kinase insert domain receptor like
mespaa	GENE	mesoderm posterior aa
smyd3	GENE	SET and MYND domain containing 3
sox17	GENE	SRY-box transcription factor 17

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Figures

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Expression

Phenotype

Fish	Conditions	Stage	Phenotype	Figure
AB + MO1-smyd3	standard conditions	50%-epiboly	blastoderm chrd expression increased distribution, abnormal	Figure 4
AB + MO1-smyd3	standard conditions	50%-epiboly	blastoderm gata5 expression increased amount, abnormal	Figure 4
AB + MO1-smyd3	standard conditions	50%-epiboly	blastoderm gata6 expression increased amount, abnormal	Figure 4
AB + MO1-smyd3	standard conditions	50%-epiboly	blastoderm gsc expression increased distribution, abnormal	Figure 4
AB + MO1-smyd3	standard conditions	50%-epiboly	blastoderm mespaa expression increased amount, abnormal	Figure 4
AB + MO1-smyd3	standard conditions	50%-epiboly	presumptive mesoderm ventral region gata2a expression decreased amount, abnormal	Figure 4
AB + MO1-smyd3	standard conditions	75%-epiboly	endodermal cell sox17 expression increased amount, abnormal	Figure 1
AB + MO1-smyd3	standard conditions	Long-pec	caudal vein plexus kdrl expression increased amount, abnormal	Figure 6
AB + MO1-smyd3	standard conditions	Long-pec	dorsal aorta kdrl expression increased amount, abnormal	Figure 6
AB + MO1-smyd3	standard conditions	Long-pec	intersegmental vessel kdrl expression increased amount, abnormal	Figure 6

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Mutations / Transgenics

Human Disease / Model

Sequence Targeting Reagents

Target	Reagent	Reagent Type
smyd3	MO1-smyd3	MRPHLNO

Fish

Fish
AB
AB + MO1-smyd3

Antibodies

Orthology

Engineered Foreign Genes

Mapping

Fittipaldi et al., 2021 ZDB-PUB-210603-22 PMID:34069776

The Lysine Methylase SMYD3 Modulates Mesendodermal Commitment during Development

Fittipaldi et al., 2021

ZDB-PUB-210603-22
PMID:34069776