PUBLICATION
New Insights into IgZ as a Maternal Transfer Ig Contributing to the Early Defense of Fish against Pathogen Infection
- Authors
- Ji, J.F., Hu, C.B., Zhang, N., Huang, X., Shao, T., Fan, D.D., Lin, A.F., Xiang, L.X., Shao, J.Z.
- ID
- ZDB-PUB-210418-3
- Date
- 2021
- Source
- Journal of immunology (Baltimore, Md. : 1950) 206(9): 2001-2014 (Journal)
- Registered Authors
- Fan, Dongdong, Lin, Aifu, Shao, Jian-zhong, Shao, Tong, Xiang, Lixin
- Keywords
- none
- MeSH Terms
-
- Aeromonas hydrophila/immunology
- Aeromonas hydrophila/physiology
- Animals
- Disease Resistance/genetics
- Disease Resistance/immunology*
- Embryo, Nonmammalian/embryology
- Embryo, Nonmammalian/immunology
- Embryo, Nonmammalian/microbiology
- Female
- Fish Diseases/immunology*
- Fish Diseases/microbiology
- Gene Expression/immunology
- Host-Pathogen Interactions/immunology
- Immunoglobulin Heavy Chains/genetics
- Immunoglobulin Heavy Chains/immunology*
- Immunoglobulin Heavy Chains/metabolism
- Immunoglobulin Isotypes/genetics
- Immunoglobulin Isotypes/immunology*
- Immunoglobulin Isotypes/metabolism
- Male
- Maternal Inheritance/genetics
- Maternal Inheritance/immunology
- Vibrio/classification
- Vibrio/immunology
- Vibrio/physiology
- Zebrafish/genetics
- Zebrafish/immunology*
- Zebrafish/microbiology
- Zebrafish Proteins/genetics
- Zebrafish Proteins/immunology*
- Zebrafish Proteins/metabolism
- Zygote/immunology
- Zygote/metabolism
- Zygote/microbiology
- PubMed
- 33858963 Full text @ J. Immunol.
Citation
Ji, J.F., Hu, C.B., Zhang, N., Huang, X., Shao, T., Fan, D.D., Lin, A.F., Xiang, L.X., Shao, J.Z. (2021) New Insights into IgZ as a Maternal Transfer Ig Contributing to the Early Defense of Fish against Pathogen Infection. Journal of immunology (Baltimore, Md. : 1950). 206(9):2001-2014.
Abstract
IgZ or its equivalent IgT is a newly discovered teleost specific Ig class that is highly specialized in mucosal immunity. However, whether this IgZ/IgT class participates in other biological processes remains unclear. In this study, we unexpectedly discovered that IgZ is highly expressed in zebrafish ovary, accumulates in unfertilized eggs, and is transmitted to offspring from eggs to zygotes. Maternally transferred IgZ in zygotes is found at the outer and inner layers of chorion, perivitelline space, periphery of embryo body, and yolk, providing different lines of defense against pathogen infection. A considerable number of IgZ+ B cells are found in ovarian connective tissues distributed between eggs. Moreover, pIgR, the transporter of IgZ, is also expressed in the ovary and colocalizes with IgZ in the zona radiata of eggs. Thus, IgZ is possibly secreted by ovarian IgZ+ B cells and transported to eggs through association with pIgR in a paracrine manner. Maternal IgZ in zygotes showed a broad bacteriostatic activity to different microbes examined, and this reactivity can be manipulated by orchestrating desired bacteria in water where parent fish live or immunizing the parent fish through vaccination. These observations suggest that maternal IgZ may represent a group of polyclonal Abs, providing protection against various environmental microbes encountered by a parent fish that were potentially high risk to offspring. To our knowledge, our findings provide novel insights into a previously unrecognized functional role of IgZ/IgT Ig in the maternal transfer of immunity in fish, greatly enriching current knowledge about this ancient Ig class.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping