PUBLICATION
Crumbs proteins stabilize the cone mosaics of photoreceptors and improve vision in zebrafish
- Authors
- Hao, Q., Zheng, M., Weng, K., Hao, Y., Zhou, Y., Lin, Y., Gao, F., Kou, Z., Kawamura, S., Yao, K., Xu, P., Chen, J., Zou, J.
- ID
- ZDB-PUB-210328-2
- Date
- 2021
- Source
- Journal of genetics and genomics = Yi chuan xue bao 48(1): 52-62 (Journal)
- Registered Authors
- Kawamura, Shoji
- Keywords
- Apical polarity, Cone mosaics, Crumbs proteins, Nearsightedness, Planar cellular polarity, Visual perception
- MeSH Terms
-
- Animals
- Cell Polarity/genetics
- Membrane Proteins*/genetics
- Membrane Proteins*/metabolism
- Retinal Cone Photoreceptor Cells*/metabolism
- Zebrafish*/genetics
- Zebrafish*/metabolism
- Zebrafish Proteins*/genetics
- Zebrafish Proteins*/metabolism
- PubMed
- 33771456 Full text @ J. Genet. Genomics
Citation
Hao, Q., Zheng, M., Weng, K., Hao, Y., Zhou, Y., Lin, Y., Gao, F., Kou, Z., Kawamura, S., Yao, K., Xu, P., Chen, J., Zou, J. (2021) Crumbs proteins stabilize the cone mosaics of photoreceptors and improve vision in zebrafish. Journal of genetics and genomics = Yi chuan xue bao. 48(1):52-62.
Abstract
Although the unique organization of vertebrate cone mosaics was first described long ago, both their underlying molecular basis and physiological significance are largely unknown. Here, we demonstrate that Crumbs proteins, the key regulators of epithelial apical polarity, establish the planar cellular polarity of photoreceptors in zebrafish. Via heterophilic Crb2a-Crb2b interactions, the apicobasal polarity protein Crb2b restricts the asymmetric planar distribution of Crb2a in photoreceptors. The planar polarized Crumbs proteins thus balance intercellular adhesions and tension between photoreceptors, thereby stabilizing the geometric organization of cone mosaics. Notably, loss of Crb2b in zebrafish induces a nearsightedness-like phenotype in zebrafish accompanied by an elongated eye axis and impairs zebrafish visual perception for predation. These data reveal a detailed mechanism for cone mosaic homeostasis via previously undiscovered apical-planar polarity coordination and propose a pathogenic mechanism for nearsightedness.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping