PUBLICATION
Slc20a1b is essential for hematopoietic stem/progenitor cell expansion in zebrafish
- Authors
- Chen, J., Li, G., Lian, J., Ma, N., Huang, Z., Li, J., Wen, Z., Zhang, W., Zhang, Y.
- ID
- ZDB-PUB-210323-15
- Date
- 2021
- Source
- Science China. Life sciences 64(12): 2186-2201 (Journal)
- Registered Authors
- Huang, Zhibin, Lian, Junwei, Ma, Ning, Wen, Zilong, Zhang, Wenqing, Zhang, Yiyue
- Keywords
- HSPC, slc20a1b, zebrafish
- Datasets
- GEO:GSE165415
- MeSH Terms
-
- Animals
- Animals, Genetically Modified
- Hematopoietic Stem Cells/physiology*
- Mutation*
- Phenotype
- Sodium-Phosphate Cotransporter Proteins, Type III/physiology*
- Zebrafish/genetics*
- Zebrafish/physiology
- Zebrafish Proteins/physiology*
- PubMed
- 33751369 Full text @ Sci. China Ser. C-Life Sci.
Citation
Chen, J., Li, G., Lian, J., Ma, N., Huang, Z., Li, J., Wen, Z., Zhang, W., Zhang, Y. (2021) Slc20a1b is essential for hematopoietic stem/progenitor cell expansion in zebrafish. Science China. Life sciences. 64(12):2186-2201.
Abstract
Hematopoietic stem and progenitor cells (HSPCs) are able to self-renew and can give rise to all blood lineages throughout their lifetime, yet the mechanisms regulating HSPC development have yet to be discovered. In this study, we characterized a hematopoiesis defective zebrafish mutant line named smu07, which was obtained from our previous forward genetic screening, and found the HSPC expansion deficiency in the mutant. Positional cloning identified that slc20a1b, which encodes a sodium phosphate cotransporter, contributed to the smu07 blood phenotype. Further analysis demonstrated that mutation of slc20a1b affects HSPC expansion through cell cycle arrest at G2/M phases in a cell-autonomous manner. Our study shows that slc20a1b is a vital regulator for HSPC proliferation in zebrafish early hematopoiesis and provides valuable insights into HSPC development.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping