PUBLICATION

PARP inhibition suppresses topoisomerase 1 poisoning induced Caspase-3 dependent cell death in zebrafish embryos

Authors
Tsikarishvili, S., Karapetian, M., Kulikova, N., Zaalishvili, G.
ID
ZDB-PUB-210312-17
Date
2021
Source
Biochemical and Biophysical Research Communications   550: 166-170 (Journal)
Registered Authors
Keywords
Caspase-3, Olaparib, PARP-1, Rubitecan, Zebrafish embryo
MeSH Terms
  • Animals
  • Apoptosis/drug effects*
  • Camptothecin/analogs & derivatives
  • Camptothecin/pharmacology
  • Caspase 3/metabolism*
  • DNA Topoisomerases, Type I/metabolism*
  • Drug Synergism
  • Phthalazines/pharmacology
  • Piperazines/pharmacology
  • Poly (ADP-Ribose) Polymerase-1/analysis
  • Poly (ADP-Ribose) Polymerase-1/chemistry
  • Poly (ADP-Ribose) Polymerase-1/metabolism*
  • Poly(ADP-ribose) Polymerase Inhibitors/pharmacology*
  • Topoisomerase I Inhibitors/pharmacology*
  • Zebrafish/embryology*
PubMed
33706100 Full text @ Biochem. Biophys. Res. Commun.
Abstract
In the present study the role of poly(ADP)ribosylation on rubitecan induced caspase dependent cell death was evaluated. We show that Top1 poisoning by rubitecan induces caspase mediated apoptosis which was reduced by PARP inhibitor olaparib in zebrafish embryo. Collectively our data introduces zebrafish as a valuable model for PARP related biomedical research.
Genes / Markers
Figures
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Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping