PUBLICATION
Labeling the Micropylar Cell in Zebrafish Whole-Mount and Cryo-sectioned Follicles
- Authors
- Dingare, C., Klemmt, P.A., Niedzwetzki, A., Lecaudey, V.
- ID
- ZDB-PUB-210220-35
- Date
- 2021
- Source
- Methods in molecular biology (Clifton, N.J.) 2218: 169-183 (Chapter)
- Registered Authors
- Lecaudey, Virginie
- Keywords
- Cryosection, Follicle cell epithelium, In situ hybridization, Micropylar cell, Micropyle, Taz, Zebrafish
- MeSH Terms
-
- Animals
- Cell Differentiation/physiology
- Female
- Fertilization/physiology
- Male
- Oocytes/metabolism
- Oocytes/physiology
- Ovarian Follicle/metabolism
- Ovarian Follicle/physiology*
- Protein Serine-Threonine Kinases/metabolism
- Signal Transduction/physiology
- Zebrafish/metabolism
- Zebrafish/physiology*
- Zebrafish Proteins/metabolism
- PubMed
- 33606231 Full text @ Meth. Mol. Biol.
Citation
Dingare, C., Klemmt, P.A., Niedzwetzki, A., Lecaudey, V. (2021) Labeling the Micropylar Cell in Zebrafish Whole-Mount and Cryo-sectioned Follicles. Methods in molecular biology (Clifton, N.J.). 2218:169-183.
Abstract
In some animal species, fertilization occurs through a funnel-like canal called the "micropyle." In teleost fishes, the micropyle is formed by a very specialized follicle cell, called the micropylar cell (MC). Very little is known about the mechanisms underlying the specification and differentiation of the MC, a unique cell among hundreds that compose the follicle cell layer. The Hippo pathway effector Taz is essential for this process and is the first reported MC marker. Here, we describe a method to identify and mark the micropylar cell following the immunostaining procedure on cryosections or combining it with the RNA in situ hybridization on whole-mount follicles.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping