PUBLICATION
Genomic Identification, Evolution and Sequence Analysis of the Heat-Shock Protein Gene Family in Buffalo
- Authors
- Rehman, S.U., Nadeem, A., Javed, M., Hassan, F.U., Luo, X., Khalid, R.B., Liu, Q.
- ID
- ZDB-PUB-201127-1
- Date
- 2020
- Source
- Genes 11(11): (Journal)
- Registered Authors
- Keywords
- Bubalus bubalis, HSP, multiple sequence alignment, phylogenetic analysis, physiochemical properties
- MeSH Terms
-
- Animals
- Buffaloes/genetics*
- Evolution, Molecular
- Heat-Shock Proteins/chemistry*
- Heat-Shock Proteins/genetics*
- Multigene Family
- Phylogeny*
- Polymorphism, Single Nucleotide
- PubMed
- 33238553 Full text @ Genes (Basel)
Citation
Rehman, S.U., Nadeem, A., Javed, M., Hassan, F.U., Luo, X., Khalid, R.B., Liu, Q. (2020) Genomic Identification, Evolution and Sequence Analysis of the Heat-Shock Protein Gene Family in Buffalo. Genes. 11(11):.
Abstract
Heat-shock proteins (HSP) are conserved chaperones crucial for protein degradation, maturation, and refolding. These adenosine triphosphate dependent chaperones were classified based on their molecular mass that ranges between 10-100 kDA, including; HSP10, HSP40, HSP70, HSP90, HSPB1, HSPD, and HSPH1 family. HSPs are essential for cellular responses and imperative for protein homeostasis and survival under stress conditions. This study performed a computational analysis of the HSP protein family to better understand these proteins at the molecular level. Physiochemical properties, multiple sequence alignment, and phylogenetic analysis were performed for 64 HSP genes in the Bubalus bubalis genome. Four genes were identified as belonging to the HSP90 family, 10 to HSP70, 39 to HSP40, 8 to HSPB, one for each HSPD, HSPH1, and HSP10, respectively. The aliphatic index was higher for HSP90 and HSP70 as compared to the HSP40 family, indicating their greater thermostability. Grand Average of hydropathicity Index values indicated the hydrophilic nature of HSP90, HSP70, and HSP40. Multiple sequence alignment indicated the presence of highly conserved consensus sequences that are plausibly significant for the preservation of structural integrity of proteins. In addition, this study has expanded our current knowledge concerning the genetic diversity and phylogenetic relatedness of HSPs of buffalo with other mammalian species. The phylogenetic tree revealed that buffalo is more closely related to Capra hircus and distantly associated with Danio rerio. Our findings provide an understanding of HSPs in buffalo at the molecular level for the first time. This study highlights functionally important HSPs and indicates the need for further investigations to better understand the role and mechanism of HSPs.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping