PUBLICATION
Somatostatin 1.1 contributes to the innate exploration of zebrafish larva
- Authors
- Quan, F.B., Desban, L., Mirat, O., Kermarquer, M., Roussel, J., Koëth, F., Marnas, H., Djenoune, L., Lejeune, F.X., Tostivint, H., Wyart, C.
- ID
- ZDB-PUB-201002-90
- Date
- 2020
- Source
- Scientific Reports 10: 15235 (Journal)
- Registered Authors
- Djenoune, Lydia, Mirat, Olivier, Tostivint, Hervé, Wyart, Claire
- Keywords
- none
- MeSH Terms
-
- Animals
- Animals, Genetically Modified
- Biomechanical Phenomena
- Exploratory Behavior/physiology
- Female
- Frameshift Mutation
- Gene Knockout Techniques
- Larva/physiology
- Locomotion/physiology
- Male
- Sequence Deletion
- Somatostatin/deficiency
- Somatostatin/genetics
- Somatostatin/physiology*
- Swimming/physiology
- Video Recording
- Zebrafish/genetics
- Zebrafish/physiology*
- Zebrafish Proteins/deficiency
- Zebrafish Proteins/genetics
- Zebrafish Proteins/physiology*
- PubMed
- 32943676 Full text @ Sci. Rep.
Citation
Quan, F.B., Desban, L., Mirat, O., Kermarquer, M., Roussel, J., Koëth, F., Marnas, H., Djenoune, L., Lejeune, F.X., Tostivint, H., Wyart, C. (2020) Somatostatin 1.1 contributes to the innate exploration of zebrafish larva. Scientific Reports. 10:15235.
Abstract
Pharmacological experiments indicate that neuropeptides can effectively tune neuronal activity and modulate locomotor output patterns. However, their functions in shaping innate locomotion often remain elusive. For example, somatostatin has been previously shown to induce locomotion when injected in the brain ventricles but to inhibit fictive locomotion when bath-applied in the spinal cord in vitro. Here, we investigated the role of somatostatin in innate locomotion through a genetic approach by knocking out somatostatin 1.1 (sst1.1) in zebrafish. We automated and carefully analyzed the kinematics of locomotion over a hundred of thousand bouts from hundreds of mutant and control sibling larvae. We found that the deletion of sst1.1 did not impact acousto-vestibular escape responses but led to abnormal exploration. sst1.1 mutant larvae swam over larger distance, at higher speed and performed larger tail bends, indicating that Somatostatin 1.1 inhibits spontaneous locomotion. Altogether our study demonstrates that Somatostatin 1.1 innately contributes to slowing down spontaneous locomotion.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping