PUBLICATION
H2O2 and Engrailed 2 paracrine activity synergize to shape the zebrafish optic tectum
- Authors
- Amblard, I., Thauvin, M., Rampon, C., Queguiner, I., Pak, V.V., Belousov, V., Prochiantz, A., Volovitch, M., Joliot, A., Vriz, S.
- ID
- ZDB-PUB-201002-212
- Date
- 2020
- Source
- Communications biology 3: 536 (Journal)
- Registered Authors
- Rampon, Christine, Vriz, Sophie
- Keywords
- none
- MeSH Terms
-
- Animals
- Homeodomain Proteins/physiology*
- Hydrogen Peroxide/metabolism*
- Nerve Tissue Proteins/physiology*
- Oxidation-Reduction
- Paracrine Communication/physiology*
- Superior Colliculi/embryology*
- Superior Colliculi/growth & development
- Zebrafish/embryology*
- Zebrafish/growth & development
- Zebrafish Proteins/physiology*
- PubMed
- 32994473 Full text @ Commun Biol
Citation
Amblard, I., Thauvin, M., Rampon, C., Queguiner, I., Pak, V.V., Belousov, V., Prochiantz, A., Volovitch, M., Joliot, A., Vriz, S. (2020) H2O2 and Engrailed 2 paracrine activity synergize to shape the zebrafish optic tectum. Communications biology. 3:536.
Abstract
Although a physiological role for redox signaling is now clearly established, the processes sensitive to redox signaling remains to be identified. Ratiometric probes selective for H2O2 have revealed its complex spatiotemporal dynamics during neural development and adult regeneration and perturbations of H2O2 levels disturb cell plasticity and morphogenesis. Here we ask whether endogenous H2O2 could participate in the patterning of the embryo. We find that perturbations of endogenous H2O2 levels impact on the distribution of the Engrailed homeoprotein, a strong determinant of midbrain patterning. Engrailed 2 is secreted from cells with high H2O2 levels and taken up by cells with low H2O2 levels where it leads to increased H2O2 production, steering the directional spread of the Engrailed gradient. These results illustrate the interplay between protein signaling pathways and metabolic processes during morphogenetic events.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping