PUBLICATION
Mitochondrial Calcium Uniporter (MCU) deficiency reveals an alternate path for Ca2+ uptake in photoreceptor mitochondria
- Authors
- Bisbach, C.M., Hutto, R.A., Poria, D., Cleghorn, W.M., Abbas, F., Vinberg, F., Kefalov, V.J., Hurley, J.B., Brockerhoff, S.E.
- ID
- ZDB-PUB-201002-211
- Date
- 2020
- Source
- Scientific Reports 10: 16041 (Journal)
- Registered Authors
- Abbas, Fatima, Brockerhoff, Susan, Hurley, James B.
- Keywords
- none
- MeSH Terms
-
- Animals
- Biological Transport
- Calcium/metabolism
- Calcium Channels/genetics
- Calcium Channels/metabolism*
- Calcium Channels/physiology
- Female
- Male
- Mice
- Mice, Inbred C57BL
- Mice, Knockout
- Mitochondria/metabolism
- Mitochondria/physiology
- Mitochondrial Membrane Transport Proteins/metabolism
- Photoreceptor Cells/metabolism
- Retinal Cone Photoreceptor Cells/metabolism*
- Retinal Rod Photoreceptor Cells/metabolism*
- Sodium-Calcium Exchanger/genetics
- Sodium-Calcium Exchanger/metabolism
- Zebrafish/metabolism
- Zebrafish Proteins/metabolism
- PubMed
- 32994451 Full text @ Sci. Rep.
Citation
Bisbach, C.M., Hutto, R.A., Poria, D., Cleghorn, W.M., Abbas, F., Vinberg, F., Kefalov, V.J., Hurley, J.B., Brockerhoff, S.E. (2020) Mitochondrial Calcium Uniporter (MCU) deficiency reveals an alternate path for Ca2+ uptake in photoreceptor mitochondria. Scientific Reports. 10:16041.
Abstract
Rods and cones use intracellular Ca2+ to regulate many functions, including phototransduction and neurotransmission. The Mitochondrial Calcium Uniporter (MCU) complex is thought to be the primary pathway for Ca2+ entry into mitochondria in eukaryotes. We investigate the hypothesis that mitochondrial Ca2+ uptake via MCU influences phototransduction and energy metabolism in photoreceptors using a mcu-/- zebrafish and a rod photoreceptor-specific Mcu-/- mouse. Using genetically encoded Ca2+ sensors to directly examine Ca2+ uptake in zebrafish cone mitochondria, we found that loss of MCU reduces but does not eliminate mitochondrial Ca2+ uptake. Loss of MCU does not lead to photoreceptor degeneration, mildly affects mitochondrial metabolism, and does not alter physiological responses to light, even in the absence of the Na+/Ca2+, K+ exchanger. Our results reveal that MCU is dispensable for vertebrate photoreceptor function, consistent with its low expression and the presence of an alternative pathway for Ca2+ uptake into photoreceptor mitochondria.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping