PUBLICATION
Polyunsaturated fatty acids synthesized by freshwater fish: A new insight to the roles of elovl2 and elovl5 in vivo
- Authors
- Sun, S., Ren, T., Li, X., Cao, X., Gao, J.
- ID
- ZDB-PUB-200905-3
- Date
- 2020
- Source
- Biochemical and Biophysical Research Communications 532(3): 414-419 (Journal)
- Registered Authors
- Keywords
- Freshwater fish, Gene knockout, LC-PUFA synthesis, Lipidomics, elovl2 and elovl5
- MeSH Terms
-
- Acetyltransferases/deficiency
- Acetyltransferases/genetics*
- Acetyltransferases/metabolism*
- Animals
- Animals, Genetically Modified
- Biosynthetic Pathways/genetics
- CRISPR-Cas Systems
- Fatty Acids, Unsaturated/biosynthesis*
- Fatty Acids, Unsaturated/chemistry
- Gene Expression Regulation, Enzymologic
- Gene Knockout Techniques
- Humans
- Lipidomics
- Liver/metabolism
- RNA, Messenger/genetics
- RNA, Messenger/metabolism
- Zebrafish/genetics*
- Zebrafish/metabolism*
- Zebrafish Proteins/deficiency
- Zebrafish Proteins/genetics*
- Zebrafish Proteins/metabolism*
- PubMed
- 32883522 Full text @ Biochem. Biophys. Res. Commun.
Citation
Sun, S., Ren, T., Li, X., Cao, X., Gao, J. (2020) Polyunsaturated fatty acids synthesized by freshwater fish: A new insight to the roles of elovl2 and elovl5 in vivo. Biochemical and Biophysical Research Communications. 532(3):414-419.
Abstract
At present, fish provide an important supply of long-chain polyunsaturated fatty acids (LC-PUFAs) for human consumption. Previous studies have shown that fatty acyl elongase 2 (elovl2) and elovl5 play important roles in fish LC-PUFA synthesis. Generally, freshwater fish have a stronger ability to synthesize LC-PUFAs than marine fish. However, the roles of elovl2, elovl5 and elovl2 + elovl5 in LC-PUFA synthesis of freshwater fish in vivo are not very clear. In this study, the elovl2 knockout zebrafish (elovl2-/-), elovl5 knockout zebrafish (elovl5-/-) and the double gene knockout zebrafish (DKO) were generated by CRISPR/Cas9 technology for the first time. Compared with wild type zebrafish (WT), elovl5-deletion zebrafish showed a significant increase in C22 PUFA content, which might be due to the up-regulation expressions of elovl4b and elovl2. elovl5 expressed at very low levels in livers of elovl2-/- relative to WT, indicating that elovl5 may be an "assistant attacker" of elovl2 in LC-PUFA synthesis of zebrafish. Moreover, there were no significant differences in levels of C18-C22 PUFAs between DKO and WT, indicating that besides elovl2 + elovl5 path, LC-PUFA synthesis in zebrafish could be performed by other paths. In addition, the hepatic lipidomic analysis results revealed that the contents of C22:6n-3 in phosphatidyl ethanolamine (PE-DHA) and PE-C22 PUFAs were more easily affected by the absence of elovl2 and elovl5. Our results suggest that the elovl2+elovl5 path is not the only path for LC-PUFA synthesis in zebrafish, and provide novel insights into the roles of elovl2 and elovl5 in LC-PUFA synthesis of freshwater fish.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping