PUBLICATION

Bsx Is Essential for Differentiation of Multiple Neuromodulatory Cell Populations in the Secondary Prosencephalon

Authors
Schredelseker, T., Veit, F., Dorsky, R.I., Driever, W.
ID
ZDB-PUB-200626-3
Date
2020
Source
Frontiers in neuroscience   14: 525 (Journal)
Registered Authors
Dorsky, Richard, Driever, Wolfgang, Schredelseker, Theresa
Keywords
aminergic neurons, basal hypothalamus, brain-specific homeobox (bsx), evolutionary conservation, neuromodulators, neuropeptides, nitrergic neurons, zebrafish
MeSH Terms
none
PubMed
32581684 Full text @ Front. Neurosci.
Abstract
The hypothalamus is characterized by great neuronal diversity, with many neuropeptides and other neuromodulators being expressed within its multiple anatomical domains. The regulatory networks directing hypothalamic development have been studied in detail, but, for many neuron types, control of differentiation is still not understood. The highly conserved Brain-specific homeobox (Bsx) transcription factor has previously been described in regulating Agrp and Npy expression in the hypothalamic arcuate nucleus (ARC) in mice. While Bsx is expressed in many more subregions of both tuberal and mamillary hypothalamus, the functions therein are not known. Using genetic analyses in zebrafish, we show that most bsx expression domains are dependent on Nkx2.1 and Nkx2.4 homeodomain transcription factors, while a subset depends on Otp. We show that the anatomical pattern of the ventral forebrain appears normal in bsx mutants, but that Bsx is necessary for the expression of many neuropeptide encoding genes, including agrp, penka, vip, trh, npb, and nts, in distinct hypothalamic anatomical domains. We also found Bsx to be critical for normal expression of two Crh family members, crhb and uts1, as well as crhbp, in the hypothalamus and the telencephalic septal region. Furthermore, we demonstrate a crucial role for Bsx in serotonergic, histaminergic and nitrergic neuron development in the hypothalamus. We conclude that Bsx is critical for the terminal differentiation of multiple neuromodulatory cell types in the forebrain.
Genes / Markers
Marker Marker Type Name
agrpGENEagouti related neuropeptide
avpGENEarginine vasopressin
bsxGENEbrain-specific homeobox
cart4GENEcocaine- and amphetamine-regulated transcript 4
crhbGENEcorticotropin releasing hormone b
crhbpGENEcorticotropin releasing hormone binding protein
dlx5aGENEdistal-less homeobox 5a
emx2GENEempty spiracles homeobox 2
fezf2GENEFEZ family zinc finger 2
foxb1aGENEforkhead box B1a
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Figures
Figure Gallery (9 images)
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Expression
Phenotype
Fish Conditions Stage Phenotype Figure
bsxm1376/m1376standard conditionsLong-pec
bsxm1376/m1376standard conditionsProtruding-mouth
bsxm1376/m1376standard conditionsProtruding-mouth
bsxm1376/m1376standard conditionsProtruding-mouth
bsxm1376/m1376standard conditionsProtruding-mouth
bsxm1376/m1376standard conditionsProtruding-mouth
bsxm1376/m1376standard conditionsProtruding-mouth
bsxm1376/m1376standard conditionsProtruding-mouth
bsxm1376/m1376standard conditionsProtruding-mouth
bsxm1376/m1376standard conditionsProtruding-mouth
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Mutations / Transgenics
Allele Construct Type Affected Genomic Region
m866
    		Small Deletion
    m1178TgTransgenic Insertion
      m1353
        		Small Deletion
        m1354
          		Small Deletion
          m1355
            		Small Deletion
            m1376
              		Indel
              sa115
                		Small Deletion
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                Human Disease / Model
                Sequence Targeting Reagents
                Fish
                Antibodies
                Orthology
                Engineered Foreign Genes
                Marker Marker Type Name
                EGFPEFGEGFP
                GFPEFGGFP
                1 - 2 of 2
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                Mapping
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                Citation
                Schredelseker, T., Veit, F., Dorsky, R.I., Driever, W. (2020) Bsx Is Essential for Differentiation of Multiple Neuromodulatory Cell Populations in the Secondary Prosencephalon. Frontiers in neuroscience. 14:525.