PUBLICATION
Short Exposure to Moderate Concentration of Alcohol During Embryonic Development Does Not Alter Gross Morphology in Zebrafish
- Authors
- Paul, I., Tsang, B., Gerlai, R.
- ID
- ZDB-PUB-200606-1
- Date
- 2020
- Source
- Zebrafish 17(4): 253-260 (Journal)
- Registered Authors
- Gerlai, Robert T.
- Keywords
- Danio rerio, alcohol, embryonic development, fetal alcohol spectrum disorder (FASD), zebrafish
- MeSH Terms
-
- Animals
- Disease Models, Animal
- Embryo, Nonmammalian/drug effects
- Embryo, Nonmammalian/embryology*
- Embryonic Development/drug effects*
- Ethanol/toxicity*
- Fetal Alcohol Spectrum Disorders/pathology
- Humans
- Time Factors
- Zebrafish/anatomy & histology*
- Zebrafish/embryology*
- PubMed
- 32493176 Full text @ Zebrafish
Citation
Paul, I., Tsang, B., Gerlai, R. (2020) Short Exposure to Moderate Concentration of Alcohol During Embryonic Development Does Not Alter Gross Morphology in Zebrafish. Zebrafish. 17(4):253-260.
Abstract
Several studies have demonstrated translational potential of the zebrafish in modeling fetal alcohol spectrum disorders (FASDs), including the less severe forms of this disease. Short exposure to even low doses of alcohol during embryonic development has been shown to disrupt behavior, alter neurochemistry, and expression of neuronal markers and glial cell phenotypes in zebrafish. However, no study to date has systematically analyzed the potential morphological effects of the short- and low-dose embryonic alcohol exposure regimen used before with zebrafish to model milder forms of human FASD. In this study, we use this previously used embryonic alcohol exposure regimen. We immerse intact zebrafish eggs of AB strain and of a genetically variable wild-type population for 2 h into 1% or 0% (vol/vol) ethanol bath at one of five developmental stages (8, 16, 24, 32, or 40 h postfertilization). At 8 days postfertilization, we quantify body length and width and eye diameter of the larvae. We report nonsignificant effects of embryonic alcohol exposure used at all developmental stages in both populations of zebrafish. Our results confirm that visual perception or motor function is unlikely to have contributed to previously reported behavioral abnormalities resulting from embryonic alcohol exposure in zebrafish.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping