PUBLICATION
Key Developmental Regulators Suggest Multiple Origins of Pancreatic Beta Cell Regeneration
- Authors
- Wang, H., Wei, X., Shi, W., He, J., Luo, L.
- ID
- ZDB-PUB-200529-1
- Date
- 2020
- Source
- Zebrafish : (Journal)
- Registered Authors
- He, Jianbo, Luo, Lingfei, Shi, Wenchao
- Keywords
- developmental regulator, regeneration, zebrafish, β cell
- MeSH Terms
- none
- PubMed
- 32460659 Full text @ Zebrafish
Citation
Wang, H., Wei, X., Shi, W., He, J., Luo, L. (2020) Key Developmental Regulators Suggest Multiple Origins of Pancreatic Beta Cell Regeneration. Zebrafish. :.
Abstract
Extensive efforts have been done to try to restore the lost β cell mass for the cure of diabetes. Animal models have been established to provide evidences of cellular origins and contextual regulators of β cell regeneration. Here, we used a zebrafish β cell ablation and regeneration model to investigate β cell neogenesis in the first few days after a near-total β cell loss. Regeneration of β cells first occurred within 7 h post-treatment. Developmental regulators such as neurod, pdx1, mnx1, and nkx2.2a were active in the regenerating β cells, while at the same time suggesting different subpopulations of regenerative cellular origins. Using Cre/loxP-based lineage tracing, we showed that intrapancreatic ductal cells resisted to give rise to regenerating β cells. Given that transdifferentiation of α cell and δ cell can regenerate β cell, here we have provided further molecular evidence highly suggesting that the regenerating β cells originate from multiple cellular origins.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping