PUBLICATION

Lineage hierarchies and stochasticity ensure the long-term maintenance of adult neural stem cells

Authors
Than-Trong, E., Kiani, B., Dray, N., Ortica, S., Simons, B., Rulands, S., Alunni, A., Bally-Cuif, L.
ID
ZDB-PUB-200520-11
Date
2020
Source
Science advances   6: eaaz5424 (Journal)
Registered Authors
Alunni, Alessandro, Bally-Cuif, Laure, Dray, Nicolas, Ortica, Sara, Than-Trong, Emmanuel
Keywords
none
MeSH Terms
  • Adult Stem Cells*
  • Animals
  • Cell Differentiation
  • Neural Stem Cells*
  • Neurogenesis
  • Telencephalon
  • Zebrafish
(all 7)
PubMed
32426477 Full text @ Sci Adv
Abstract
The cellular basis and extent of neural stem cell (NSC) self-renewal in adult vertebrates, and their heterogeneity, remain controversial. To explore the functional behavior and dynamics of individual NSCs, we combined genetic lineage tracing, quantitative clonal analysis, intravital imaging, and global population assessments in the adult zebrafish telencephalon. Our results are compatible with a model where adult neurogenesis is organized in a hierarchy in which a subpopulation of deeply quiescent reservoir NSCs with long-term self-renewal potential generate, through asymmetric divisions, a pool of operational NSCs activating more frequently and taking stochastic fates biased toward neuronal differentiation. Our data further suggest the existence of an additional, upstream, progenitor population that supports the continuous generation of new reservoir NSCs, thus contributing to their overall expansion. Hence, we propose that the dynamics of vertebrate neurogenesis relies on a hierarchical organization where growth, self-renewal, and neurogenic functions are segregated between different NSC types.
Genes / Markers
Marker Marker Type Name
mitfaGENEmelanocyte inducing transcription factor a
mpv17GENEmitochondrial inner membrane protein MPV17
1 - 2 of 2
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Figures
Figure Gallery (6 images)
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Expression
Phenotype
No data available
Mutations / Transgenics
Allele Construct Type Affected Genomic Region
a9
    		Complex
    cz1701TgTransgenic Insertion
      knu2TgTransgenic Insertion
        mi2001TgTransgenic Insertion
          mi2004TgTransgenic Insertion
            nns17TgTransgenic Insertion
              w2
                		Point Mutation
                1 - 7 of 7
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                Human Disease / Model
                No data available
                Sequence Targeting Reagents
                No data available
                Fish
                No data available
                Antibodies
                Orthology
                No data available
                Engineered Foreign Genes
                Marker Marker Type Name
                EGFPEFGEGFP
                GFPEFGGFP
                mCherryEFGmCherry
                RFPEFGRFP
                TomatoEFGTomato
                1 - 5 of 5
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                Mapping
                No data available
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                Citation
                Than-Trong, E., Kiani, B., Dray, N., Ortica, S., Simons, B., Rulands, S., Alunni, A., Bally-Cuif, L. (2020) Lineage hierarchies and stochasticity ensure the long-term maintenance of adult neural stem cells. Science advances. 6:eaaz5424.