PUBLICATION

Zebrafish can regenerate endoskeleton in larval pectoral fin but the regenerative ability declines

Authors
Yoshida, K., Kawakami, K., Abe, G., Tamura, K.
ID
ZDB-PUB-200519-6
Date
2020
Source
Developmental Biology   463(2): 110-123 (Journal)
Registered Authors
Kawakami, Koichi
Keywords
Endoskeleton, Epimorphic, Fin, Regeneration, Zebrafish
MeSH Terms
  • Animal Fins/physiology*
  • Animals
  • Gene Expression Regulation*
  • Homeodomain Proteins/biosynthesis*
  • Homeodomain Proteins/genetics
  • Regeneration*
  • Zebrafish/genetics
  • Zebrafish/metabolism*
  • Zebrafish Proteins/biosynthesis*
  • Zebrafish Proteins/genetics
(all 10)
PubMed
32422142 Full text @ Dev. Biol.
Abstract
We show for the first time endoskeletal regeneration in the developing pectoral fin of zebrafish. The developing pectoral fin contains an aggregation plate of differentiated chondrocytes (endochondral disc; primordium for endoskeletal components, proximal radials). The endochondral disc can be regenerated after amputation in the middle of the disc. The regenerated disc sufficiently forms endoskeletal patterns. Early in the process of regenerating the endochondral disc, epithelium with apical ectodermal ridge (AER) marker expression rapidly covers the amputation plane, and mesenchymal cells start to actively proliferate. Taken together with re-expression of a blastema marker gene, msxb, and other developmental genes, it is likely that regeneration of the endochondral disc recaptures fin development as epimorphic limb regeneration does. The ability of endoskeletal regeneration declines during larval growth, and adult zebrafish eventually lose the ability to regenerate endoskeletal components such that amputated endoskeletons become enlarged. Endoskeletal regeneration in the zebrafish pectoral fin will serve as a new model system for successful appendage regeneration in mammals.
Genes / Markers
No data available
Figures
Figure Gallery (9 images)
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Expression
Phenotype
Fish Conditions Stage Phenotype Figure
WTamputation: pectoral finAdult
1 - 1 of 1
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Mutations / Transgenics
Allele Construct Type Affected Genomic Region
js301TgTransgenic Insertion
    nkhspGFFDMC131AEtTransgenic Insertion
      nknkgsaizGFFM1116AGtTransgenic Insertion
        nkuasgfp1aTgTransgenic Insertion
          vu234TgTransgenic Insertion
            1 - 5 of 5
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            Human Disease / Model
            No data available
            Sequence Targeting Reagents
            No data available
            Fish
            Fish
            WT
            1 - 1 of 1
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            Antibodies
            Name Type Antigen Genes Isotypes Host Organism
            Ab10-casp3polyclonal
              		IgGRabbit
              Ab14-h3monoclonal
                		IgG1Mouse
                1 - 2 of 2
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                Orthology
                No data available
                Engineered Foreign Genes
                Marker Marker Type Name
                EGFPEFGEGFP
                GAL4FFEFGGAL4FF
                mRFPEFGmRFP
                1 - 3 of 3
                Show
                Mapping
                No data available
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                Citation
                Yoshida, K., Kawakami, K., Abe, G., Tamura, K. (2020) Zebrafish can regenerate endoskeleton in larval pectoral fin but the regenerative ability declines. Developmental Biology. 463(2):110-123.