PUBLICATION
CRISPR mutation in lysine (K)-specific demethylase 1a (kdm1a/LSD1) does not produce defects to skeletal development in maternal zygotic mutant zebrafish larvae
- Authors
- DeLaurier A., Wiggins K.J., Mishoe Hernandez L., Loyo Rosado F., Pugh, K., Shabdue, C.
- ID
- ZDB-PUB-200515-15
- Date
- 2020
- Source
- ZebraShare : (Unpublished)
- Registered Authors
- DeLaurier, April
- Keywords
- none
- MeSH Terms
- none
- PubMed
- none
Citation
DeLaurier A., Wiggins K.J., Mishoe Hernandez L., Loyo Rosado F., Pugh, K., Shabdue, C. (2020) CRISPR mutation in lysine (K)-specific demethylase 1a (kdm1a/LSD1) does not produce defects to skeletal development in maternal zygotic mutant zebrafish larvae. ZebraShare. .
Abstract
CRISPR-Cas9 technology was used to generate a mutation in exon 1 of kdm1a (LSD1). A 14 bp deletion was generated, causing missense and a premature stop codon predicted to cause a truncated protein. Mutants are viable and able to reproduce. Maternal zygotic mutants were generated and examined for skeletal phenotypes using Alician Blue and Alizarin Red dyes. No obvious skeletal defects were observed in fish at 4 days post-fertilization.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping