PUBLICATION
Retinoic Acid Organizes the Zebrafish Vagus Motor Topographic Map via Spatiotemporal Coordination of Hgf/Met Signaling
- Authors
- Isabella, A.J., Barsh, G.R., Stonick, J.A., Dubrulle, J., Moens, C.B.
- ID
- ZDB-PUB-200422-2
- Date
- 2020
- Source
- Developmental Cell 53(3): 344-357.e5 (Journal)
- Registered Authors
- Dubrulle, Julien, Moens, Cecilia
- Keywords
- axon guidance, hepatocyte growth factor, met, motor neuron, nervous system development, pharyngeal arch, retinoic acid, topographic map, vagus, zebrafish
- Datasets
- GEO:GSE135780, GEO:GSE135781
- MeSH Terms
-
- Animals
- Branchial Region/drug effects
- Branchial Region/physiology
- Gene Expression Regulation, Developmental/drug effects*
- Hepatocyte Growth Factor/genetics
- Hepatocyte Growth Factor/metabolism*
- Keratolytic Agents/pharmacology
- Proto-Oncogene Proteins c-met/genetics
- Proto-Oncogene Proteins c-met/metabolism*
- Signal Transduction
- Spatio-Temporal Analysis
- Tretinoin/pharmacology*
- Vagus Nerve/drug effects
- Vagus Nerve/physiology*
- Zebrafish/physiology*
- Zebrafish Proteins/genetics
- Zebrafish Proteins/metabolism*
- PubMed
- 32302545 Full text @ Dev. Cell
Citation
Isabella, A.J., Barsh, G.R., Stonick, J.A., Dubrulle, J., Moens, C.B. (2020) Retinoic Acid Organizes the Zebrafish Vagus Motor Topographic Map via Spatiotemporal Coordination of Hgf/Met Signaling. Developmental Cell. 53(3):344-357.e5.
Abstract
Information flow through neural circuits often requires their organization into topographic maps in which the positions of cell bodies and synaptic targets correspond. To understand how topographic map development is controlled, we examine the mechanism underlying targeting of vagus motor axons to the pharyngeal arches in zebrafish. We reveal that retinoic acid organizes topography by specifying anterior-posterior identity in vagus motor neurons. We then show that chemoattractant signaling between Hgf and Met is required for vagus innervation of the pharyngeal arches. Finally, we find that retinoic acid controls the spatiotemporal dynamics of Hgf/Met signaling to coordinate axon targeting with the developmental progression of the pharyngeal arches and show that experimentally altering the timing of Hgf/Met signaling is sufficient to redirect axon targeting and disrupt the topographic map. These findings establish a mechanism of topographic map development in which the regulation of chemoattractant signaling in space and time guides axon targeting.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping