PUBLICATION
The extracellular and intracellular regions of Crb2a play distinct roles in guiding the formation of the apical zonula adherens
- Authors
- Lin, Y., Weng, K., Liang, J., Zhou, Y., Hao, Q., Hao, Y., Yao, K., Zou, J.
- ID
- ZDB-PUB-200212-16
- Date
- 2020
- Source
- Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie 125: 109942 (Journal)
- Registered Authors
- Zou, Jian
- Keywords
- Adherens junctions, Crumbs, Outer limiting membrane, Punctum adherens, Zonula adherens
- MeSH Terms
-
- Adherens Junctions/physiology*
- Animals
- Epithelium/metabolism
- Extracellular Space/physiology
- Intracellular Space/physiology
- Lens, Crystalline/metabolism
- Membrane Proteins/genetics
- Membrane Proteins/metabolism*
- Morphogenesis/physiology*
- Mutation
- Retina/metabolism
- Zebrafish
- Zebrafish Proteins/genetics
- Zebrafish Proteins/metabolism*
- PubMed
- 32044715 Full text @ Biomed. Pharmacother.
Citation
Lin, Y., Weng, K., Liang, J., Zhou, Y., Hao, Q., Hao, Y., Yao, K., Zou, J. (2020) The extracellular and intracellular regions of Crb2a play distinct roles in guiding the formation of the apical zonula adherens. Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie. 125:109942.
Abstract
The transmembrane protein Crumbs (Crb), a key regulator of apical polarity, has a known involvement in establishment of the apical zonula adherens in epithelia, although the precise mechanism remains elusive. The zonula adherens are required to maintain the integrity and orderly arrangement of epithelia. Loss of the zonula adherens leads to morphogenetic defects in the tissues derived from epithelium. In this study, we revealed that the intracellular tail of Crb2a promoted the apical distribution of adherens junctions (AJs) in zebrafish retinal and lens epithelia, but caused assembly into unstable punctum adherens-like adhesion plaques. The extracellular region of Crb2a guided the transformation of AJs from the punctum adherens into stable zonula adherens. Accordingly, a truncated form of Crb2a lacking the extracellular region (Crb2aΔEX) could only partially rescue the retinal patterning defects in crb2a null mutant zebrafish (crb2am289). By contrast, constitutive over-expression of Crb2aΔEX disrupted the integrity of the outer limiting membrane in photoreceptors, which is derived from the zonula adherens of the retinal neuroepithelium. This study demonstrated that both the extracellular region and the intracellular tail of Crb2a are required to guide the formation of the apical zonula adherens.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping