PUBLICATION
Wdr26 regulates nuclear condensation in developing erythroblasts
- Authors
- Zhen, R., Moo, C., Zhao, Z., Chen, M., Feng, H., Zheng, X., Zhang, L., Shi, J., Chen, C.
- ID
- ZDB-PUB-200118-12
- Date
- 2020
- Source
- Blood 135: 208-219 (Journal)
- Registered Authors
- Keywords
- none
- MeSH Terms
-
- Animals
- Cell Differentiation*
- Cell Nucleus/genetics
- Cell Nucleus/metabolism*
- Erythroblasts/cytology
- Erythroblasts/physiology*
- Erythropoiesis*
- Mice
- Ubiquitin-Protein Ligases/metabolism
- Ubiquitination
- Zebrafish/genetics
- Zebrafish/growth & development
- Zebrafish/metabolism*
- Zebrafish Proteins/genetics
- Zebrafish Proteins/metabolism*
- PubMed
- 31945154 Full text @ Blood
Citation
Zhen, R., Moo, C., Zhao, Z., Chen, M., Feng, H., Zheng, X., Zhang, L., Shi, J., Chen, C. (2020) Wdr26 regulates nuclear condensation in developing erythroblasts. Blood. 135:208-219.
Abstract
Mammalian red blood cells lack nuclei. The molecular mechanisms underlying erythroblast nuclear condensation and enucleation, however, remain poorly understood. Here we show that Wdr26, a gene upregulated during terminal erythropoiesis, plays an essential role in regulating nuclear condensation in differentiating erythroblasts. Loss of Wdr26 induces anemia in zebrafish and enucleation defects in mouse erythroblasts because of impaired erythroblast nuclear condensation. As part of the glucose-induced degradation-deficient ubiquitin ligase complex, Wdr26 regulates the ubiquitination and degradation of nuclear proteins, including lamin B. Failure of lamin B degradation blocks nuclear opening formation leading to impaired clearance of nuclear proteins and delayed nuclear condensation. Collectively, our study reveals an unprecedented role of an E3 ubiquitin ligase in regulating nuclear condensation and enucleation during terminal erythropoiesis. Our results provide mechanistic insights into nuclear protein homeostasis and vertebrate red blood cell development.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping