PUBLICATION
In vivo analysis of renal epithelial cells in zebrafish
- Authors
- Li, Y., Xu, W., Jerman, S., Sun, Z.
- ID
- ZDB-PUB-190909-1
- Date
- 2019
- Source
- Methods in cell biology 154: 163-181 (Chapter)
- Registered Authors
- Sun, Zhaoxia
- Keywords
- Apical-basal polarity, CRISPR, Cilia, Epithelial cells, Kidney, Zebrafish
- MeSH Terms
-
- Animals
- Base Sequence
- CRISPR-Associated Protein 9/genetics
- CRISPR-Associated Protein 9/metabolism
- CRISPR-Cas Systems
- Cell Polarity
- Cilia/metabolism
- Cilia/ultrastructure
- Embryo, Nonmammalian/anatomy & histology
- Embryo, Nonmammalian/cytology*
- Embryo, Nonmammalian/metabolism
- Epithelial Cells/cytology*
- Epithelial Cells/metabolism
- Female
- Gene Editing/methods*
- Immunohistochemistry/methods*
- Kidney/cytology*
- Kidney/embryology
- Kidney/metabolism
- Male
- Organogenesis/genetics
- RNA, Guide, Kinetoplastida/genetics
- RNA, Guide, Kinetoplastida/metabolism
- Zebrafish
- Zebrafish Proteins/deficiency
- Zebrafish Proteins/genetics*
- PubMed
- 31493817 Full text @ Meth. Cell. Biol.
Citation
Li, Y., Xu, W., Jerman, S., Sun, Z. (2019) In vivo analysis of renal epithelial cells in zebrafish. Methods in cell biology. 154:163-181.
Abstract
The zebrafish kidney has been used effectively for studying kidney development, repair and disease. New gene editing capability makes it a more versatile in vivo vertebrate model system to investigate renal epithelial cells in their native environment. In this chapter we focus on dissecting gene function in basic cellular biology of renal epithelial cells, including lumen formation and cell polarity, in intact zebrafish embryos.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping