PUBLICATION

Tissue-Specific Transcriptomes Reveal Gene Expression Trajectories in Two Maturing Skin Epithelial Layers in Zebrafish Embryos

Authors
Cokus, S.J., De La Torre, M., Medina, E.F., Rasmussen, J.P., Ramirez-Gutierrez, J., Sagasti, A., Wang, F.
ID
ZDB-PUB-190822-2
Date
2019
Source
G3 (Bethesda)   9(10): 3439-3452 (Journal)
Registered Authors
Keywords
epithelia, keratin, skin development, transcriptome, zebrafish
Datasets
GEO:GSE132304
MeSH Terms
  • Animals
  • Embryonic Development/genetics*
  • Epithelium/embryology*
  • Gene Expression Profiling
  • Gene Expression Regulation, Developmental
  • Organogenesis/genetics*
  • Phenotype
  • Transcriptome*
  • Zebrafish/embryology*
  • Zebrafish/genetics*
(all 10)
PubMed
31431477 Full text @ G3 (Bethesda)
Abstract
Epithelial cells are the building blocks of many organs, including skin. The vertebrate skin initially consists of two epithelial layers, the outer periderm and inner basal cell layers, which have distinct properties, functions, and fates. The embryonic periderm ultimately disappears during development, whereas basal cells proliferate to form the mature, stratified epidermis. Although much is known about mechanisms of homeostasis in mature skin, relatively little is known about the two cell types in pre-stratification skin. To define the similarities and distinctions between periderm and basal skin epithelial cells, we purified them from zebrafish at early development stages and deeply profiled their gene expression. These analyses identified groups of genes whose tissue enrichment changed at each stage, defining gene flow dynamics of maturing vertebrate epithelia. At each of 52 and 72 hours post-fertilization (hpf), more than 60% of genes enriched in skin cells were similarly expressed in both layers, indicating that they were common epithelial genes, but many others were enriched in one layer or the other. Both expected and novel genes were enriched in periderm and basal cell layers. Genes encoding extracellular matrix, junctional, cytoskeletal, and signaling proteins were prominent among those distinguishing the two epithelial cell types. In situ hybridization and BAC transgenes confirmed our expression data and provided new tools to study zebrafish skin. Collectively, these data provide a resource for studying common and distinguishing features of maturing epithelia.
Genes / Markers
Figures
Figure Gallery (1 images)
Show all Figures
Expression
Phenotype
No data available
Mutations / Transgenics
Allele Construct Type Affected Genomic Region
la203TgTransgenic Insertion
    la212TgTransgenic Insertion
      la213TgTransgenic Insertion
        ncc100TgTransgenic Insertion
          rw0144TgTransgenic Insertion
            1 - 5 of 5
            Show
            Human Disease / Model
            No data available
            Sequence Targeting Reagents
            No data available
            Fish
            Fish
            la203Tg; ncc100Tg
            la212Tg; rw0144Tg
            la213Tg; rw0144Tg
            WT
            1 - 4 of 4
            Show
            Antibodies
            No data available
            Orthology
            Engineered Foreign Genes
            Marker Marker Type Name
            DsRedEFGDsRed
            EGFPEFGEGFP
            GAL4FFEFGGAL4FF
            mCherryEFGmCherry
            1 - 4 of 4
            Show
            Mapping
            Your Input Welcome
            We welcome your input and comments. Please use this form to recommend updates to the information in ZFIN. We appreciate as much detail as possible and references as appropriate. We will review your comments promptly.
            Citation
            Cokus, S.J., De La Torre, M., Medina, E.F., Rasmussen, J.P., Ramirez-Gutierrez, J., Sagasti, A., Wang, F. (2019) Tissue-Specific Transcriptomes Reveal Gene Expression Trajectories in Two Maturing Skin Epithelial Layers in Zebrafish Embryos. G3 (Bethesda). 9(10):3439-3452.