PUBLICATION
Investigating the role of prostaglandin receptor isoform EP4b in zebrafish ovulation
- Authors
- Baker, S.J.C., Van Der Kraak, G.
- ID
- ZDB-PUB-190729-6
- Date
- 2019
- Source
- General and comparative endocrinology 283: 113228 (Journal)
- Registered Authors
- Van Der Kraak, Glen
- Keywords
- Zebrafish, ovulation, prostaglandin, prostaglandin receptor isoforms, reproduction
- MeSH Terms
-
- Animals
- Domperidone/pharmacology
- Drug Combinations
- Female
- Gene Expression Regulation/drug effects
- Gonadotropin-Releasing Hormone/pharmacology
- Hydroxyprogesterones/pharmacology
- Ovary/drug effects
- Ovary/metabolism
- Ovulation/drug effects
- Ovulation/physiology*
- Protein Isoforms/genetics
- Protein Isoforms/metabolism
- RNA, Messenger/genetics
- RNA, Messenger/metabolism
- Receptors, Prostaglandin/genetics
- Receptors, Prostaglandin/metabolism*
- Time Factors
- Zebrafish/genetics
- Zebrafish/metabolism*
- Zebrafish Proteins/genetics
- Zebrafish Proteins/metabolism*
- PubMed
- 31348957 Full text @ Gen. Comp. Endocrinol.
Citation
Baker, S.J.C., Van Der Kraak, G. (2019) Investigating the role of prostaglandin receptor isoform EP4b in zebrafish ovulation. General and comparative endocrinology. 283:113228.
Abstract
Prostaglandins (PGs) are a class of fatty acid-derived hormones that play an essential role in the regulation of ovulation of teleosts. This study investigated the various isoforms of ovarian PG receptors in the zebrafish ovary and their role in ovulation. Using real time qPCR, six PG receptor isoforms (ptger1a, ptger1b, ptger2a, ptger4a, ptger4b, and ptgfr) were shown to be expressed in the ovary. Only the PG receptor isoform ptger4b was upregulated at the time of ovulation in vivo, or following treatment in vivo with Ovaprim, which contains a gonadotropin releasing hormone analogue and a dopamine receptor antagonist and stimulates ovulation. Treatment of full-grown follicles with the maturation-inducing hormone 17α,20β-dihydroxy-4-pregnen-3-one (17,20βP) in vitro also induced expression of EP4b mRNA. Females ovulate in vivo after injection with Ovaprim, or injection with Ovaprim and inhibitors of EP1 (ONO-8130) or EP2 (TG4-155) function; they do not ovulate when injected with Ovaprim and an EP4 inhibitor (GW237368x). These findings suggest that the EP4 receptor, in particular the EP4b isoform, is essential for ovulation.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping