PUBLICATION

Skeletal mineralisation in association with type X collagen expression is an ancestral feature for jawed vertebrates

Authors
Debiais-Thibaud, M., Simion, P., Ventéo, S., Muñoz, D., Marcellini, S., Mazan, S., Haitina, T.
ID
ZDB-PUB-190705-4
Date
2019
Source
Molecular Biology and Evolution   36(10): 2265-2276 (Journal)
Registered Authors
Debiais-Thibaud, Mélanie
Keywords
chondrichthyan, mineralisation, scales, teeth, type X collagen
MeSH Terms
  • Animals
  • Calcification, Physiologic/genetics*
  • Collagen Type X/genetics*
  • Collagen Type X/metabolism
  • Elasmobranchii/genetics*
  • Elasmobranchii/metabolism
  • Gene Duplication
  • Phylogeny
  • Synteny
(all 9)
PubMed
31270539 Full text @ Mol Bio Evol
Abstract
In order to characterize the molecular bases of mineralising cell evolution, we targeted type X collagen, a non-fibrillar network forming collagen encoded by the Col10a1 gene. It is involved in the process of endochondral ossification in ray-finned fishes and tetrapods (Osteichthyes), but until now unknown in cartilaginous fishes (Chondrichthyes). We show that holocephalans and elasmobranchs have respectively five and six tandemly duplicated Col10a1 gene copies that display conserved genomic synteny with osteichthyan Col10a1 genes. All Col10a1 genes in the catshark Scyliorhinus canicula are expressed in ameloblasts and/or odontoblasts of teeth and scales, during the stages of extracellular matrix protein secretion and mineralisation. Only one duplicate is expressed in the endoskeletal (vertebral) mineralising tissues. We also show that the expression of type X collagen is present in teeth of two osteichthyans, the zebrafish Danio rerio and the western clawed frog Xenopus tropicalis, indicating an ancestral jawed vertebrate involvement of type X collagen in odontode formation. Our findings push the origin of Col10a1 gene prior to the divergence of osteichthyans and chondrichthyans, and demonstrate its ancestral association with mineralisation of both the odontode skeleton and the endoskeleton.
Genes / Markers
Marker Marker Type Name
col10a1aGENEcollagen, type X, alpha 1a
col10a1bGENEcollagen, type X, alpha 1b
col8a1aGENEcollagen, type VIII, alpha 1a
col8a1bGENEcollagen, type VIII, alpha 1b
col8a2GENEcollagen, type VIII, alpha 2
frkGENEfyn-related Src family tyrosine kinase
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Figures
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Expression
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay Figure
col10a1aWTstandard conditionsDay 5ISH
col10a1aWTstandard conditionsDay 4ISH
col10a1aWTstandard conditionsDay 6ISH
col10a1aWTstandard conditionsDay 4ISH
col10a1aWTstandard conditionsDay 5ISH
col10a1aWTstandard conditionsDay 6ISH
col10a1aWTstandard conditionsDay 5ISH
col10a1aWTstandard conditionsDay 4ISH
col10a1aWTstandard conditionsDay 6ISH
col10a1aWTstandard conditionsDay 4ISH
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Phenotype
No data available
Mutations / Transgenics
No data available
Human Disease / Model
No data available
Sequence Targeting Reagents
No data available
Fish
Fish
WT
1 - 1 of 1
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Antibodies
No data available
Orthology
Gene Orthology
col10a1a
col10a1b
frk
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Engineered Foreign Genes
No data available
Mapping
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Citation
Debiais-Thibaud, M., Simion, P., Ventéo, S., Muñoz, D., Marcellini, S., Mazan, S., Haitina, T. (2019) Skeletal mineralisation in association with type X collagen expression is an ancestral feature for jawed vertebrates. Molecular Biology and Evolution. 36(10):2265-2276.