PUBLICATION
Hydroxycholesterol binds and enhances the anti-viral activities of zebrafish monomeric c-reactive protein isoforms
- Authors
- Bello-Perez, M., Falco, A., Novoa, B., Perez, L., Coll, J.
- ID
- ZDB-PUB-190118-7
- Date
- 2019
- Source
- PLoS One 14: e0201509 (Journal)
- Registered Authors
- Novoa, Beatriz
- Keywords
- none
- MeSH Terms
-
- Animals
- C-Reactive Protein/immunology*
- Cell Line
- Fish Diseases*/immunology
- Fish Diseases*/virology
- Hydroxycholesterols/immunology*
- Rhabdoviridae/immunology*
- Rhabdoviridae Infections*/immunology
- Rhabdoviridae Infections*/veterinary
- Rhabdoviridae Infections*/virology
- Zebrafish*/immunology
- Zebrafish*/virology
- Zebrafish Proteins/immunology*
- PubMed
- 30653529 Full text @ PLoS One
Citation
Bello-Perez, M., Falco, A., Novoa, B., Perez, L., Coll, J. (2019) Hydroxycholesterol binds and enhances the anti-viral activities of zebrafish monomeric c-reactive protein isoforms. PLoS One. 14:e0201509.
Abstract
C-reactive proteins (CRPs) are among the faster acute-phase inflammation-responses proteins encoded by one gene (hcrp) in humans and seven genes (crp1-7) in zebrafish (Danio rerio) with importance in bacterial and viral infections. In this study, we described novel preferential bindings of 25-hydroxycholesterol (25HOCh) to CRP1-7 compared with other lipids and explored the antiviral effects of both 25HOCh and CRP1-7 against spring viremia carp virus (SVCV) infection in zebrafish. Both in silico and in vitro results confirmed the antiviral effect of 25HOCh and CRP1-7 interactions, thereby showing that the crosstalk between them differed among the zebrafish isoforms. The presence of oxidized cholesterols in human atherosclerotic plaques amplifies the importance that similar interactions may occur for vascular and/or neurodegenerative diseases during viral infections. In this context, the zebrafish model offers a genetic tool to further investigate these interactions.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping