PUBLICATION

Dietary nucleotides can directly stimulate the immunity of zebrafish independent of the intestinal microbiota

Authors
Guo, X., Li, J., Ran, C., Wang, A., Xie, M., Xie, Y., Ding, Q., Zhang, Z., Yang, Y., Zhou, Z.
ID
ZDB-PUB-181228-8
Date
2018
Source
Fish & shellfish immunology   86: 1064-1071 (Journal)
Registered Authors
Ding, Qianwen, Li, Jie, Ran, Chao, Xie, Yadong, Yang, Yalin, Zhang, Zhen, Zhou, Zhigang
Keywords
Innate immune response, Intestinal microbiota, Nucleotides, Zebrafish
MeSH Terms
  • Aeromonas hydrophila/physiology
  • Animal Feed/analysis
  • Animals
  • Diet/veterinary*
  • Fish Diseases/immunology
  • Fish Diseases/microbiology
  • Gastrointestinal Microbiome*
  • Germ-Free Life
  • Gram-Negative Bacterial Infections/immunology
  • Head Kidney/immunology
  • Immunity, Innate/drug effects
  • Nucleotides/pharmacology*
  • Zebrafish/immunology*
  • Zebrafish/microbiology*
(all 14)
PubMed
30590163 Full text @ Fish Shellfish Immunol.
Abstract
In this study, we firstly tested the effects of dietary nucleotides on the disease resistance and innate immunity of zebrafish. Further, we investigated the role of intestinal microbiota in the nucleotides-induced immunostimulatory effect by using a germ-free zebrafish model and microbiota transfer technique. Fish were fed control or nucleotides (NT)-supplemented diets (at 0.05%,0.1%, 0.15% or 0.2%, m/m) for 4 weeks, followed by immersion challenge with Aeromonas hydrophila NJ-1. The results showed that 0.1% NT group enhanced the resistance of zebrafish against A. hydrophila infection. We further observed that the relative expressions of mucin, claudin16, occlusin1, hepcidin, defensin beta-like, myeloperoxidase (Mpo), and serum amyloid A (Saa) increased in the 0.1% NT group compared with control (P < 0.05), indicating that dietary nucleotides enhanced the physical barrier and mucosal immunity in the intestine of zebrafish. Moreover, ROS level in the head kidney was significantly increased in NT fed zebrafish versus control (P < 0.05), indicating enhanced systematic immunity. Furthermore, dietary NT significantly elevated the relative expressions of mpo, saa and the ROS activity in germ-free zebrafish, while germ-free zebrafish colonized with NT-altered microbiota had no significant difference in the relative expressions of mpo, saa and the ROS activity compared with the control microbiota-colonized fish, suggesting that the immunostimulatory effect of dietary NT is mediated by direct action of NT and does not involve the microbiota. Consistently, dietary NT can protect germ-free zebrafish from pathogenic infection, whereas germ-free zebrafish colonized with NT microbiota showed no difference in disease resistance compared with control microbiota colonized counterparts. Together, these results indicated that the immunostimulatory and disease protection effect of dietary nucleotides in zebrafish was mediated by direct action of the nucleotides, and does not involve the intestinal microbiota.
Genes / Markers
Marker Marker Type Name
cldn12GENEclaudin 12
cldn15aGENEclaudin 15a
cldn3dGENEclaudin 3d
defbl1GENEdefensin, beta-like 1
hampGENEhepcidin antimicrobial peptide
lyzGENElysozyme
muc2.2GENEmucin 2.2, oligomeric mucus/gel-forming
oclnbGENEoccludin b
rps11GENEribosomal protein S11
rps11bGENEribosomal protein S11b
1 - 10 of 10
Show
Figures
No images available
Show all Figures
Expression
Phenotype
Fish Conditions Stage Phenotype Figure
WTchemical treatment by diet: guanosine 5'-monophosphate, chemical treatment by diet: IMP, chemical treatment by diet: adenosine 5'-phosphate, chemical treatment by diet: uridine 5'-monophosphate, chemical treatment by diet: cytidine 5'-monophosphateDays 30-44 to Days 45-89
WTchemical treatment by diet: guanosine 5'-monophosphate, chemical treatment by diet: IMP, chemical treatment by diet: adenosine 5'-phosphate, chemical treatment by diet: uridine 5'-monophosphate, chemical treatment by diet: cytidine 5'-monophosphateDays 30-44 to Days 45-89
WTchemical treatment by diet: guanosine 5'-monophosphate, chemical treatment by diet: IMP, chemical treatment by diet: adenosine 5'-phosphate, chemical treatment by diet: uridine 5'-monophosphate, chemical treatment by diet: cytidine 5'-monophosphateDays 30-44 to Days 45-89
WTchemical treatment by diet: guanosine 5'-monophosphate, chemical treatment by diet: IMP, chemical treatment by diet: adenosine 5'-phosphate, chemical treatment by diet: uridine 5'-monophosphate, chemical treatment by diet: cytidine 5'-monophosphateDays 30-44 to Days 45-89
WTchemical treatment by diet: guanosine 5'-monophosphate, chemical treatment by diet: IMP, chemical treatment by diet: adenosine 5'-phosphate, chemical treatment by diet: uridine 5'-monophosphate, chemical treatment by diet: cytidine 5'-monophosphateDays 30-44 to Days 45-89
WTchemical treatment by diet: guanosine 5'-monophosphate, chemical treatment by diet: IMP, chemical treatment by diet: adenosine 5'-phosphate, chemical treatment by diet: uridine 5'-monophosphate, chemical treatment by diet: cytidine 5'-monophosphateDays 30-44 to Days 45-89
WTchemical treatment by diet: guanosine 5'-monophosphate, chemical treatment by diet: IMP, chemical treatment by diet: adenosine 5'-phosphate, chemical treatment by diet: uridine 5'-monophosphate, chemical treatment by diet: cytidine 5'-monophosphateDays 30-44 to Days 45-89
WTbacterial treatment by exposure to environment: Aeromonas hydrophilaDays 30-44 to Days 45-89
WTgerm freeDay 5
WTbacterial treatment by exposure to environment: Aeromonas hydrophila, germ freeDay 5 to Day 6
1 - 10 of 13
Show
Mutations / Transgenics
No data available
Human Disease / Model
No data available
Sequence Targeting Reagents
No data available
Fish
Fish
WT
1 - 1 of 1
Show
Antibodies
Orthology
Engineered Foreign Genes
No data available
Mapping
Your Input Welcome
We welcome your input and comments. Please use this form to recommend updates to the information in ZFIN. We appreciate as much detail as possible and references as appropriate. We will review your comments promptly.
Citation
Guo, X., Li, J., Ran, C., Wang, A., Xie, M., Xie, Y., Ding, Q., Zhang, Z., Yang, Y., Zhou, Z. (2018) Dietary nucleotides can directly stimulate the immunity of zebrafish independent of the intestinal microbiota. Fish & shellfish immunology. 86:1064-1071.