PUBLICATION

Planar cell polarity signalling coordinates heart tube remodelling through tissue-scale polarisation of actomyosin activity

Authors
Merks, A.M., Swinarski, M., Meyer, A.M., Müller, N.V., Özcan, I., Donat, S., Burger, A., Gilbert, S., Mosimann, C., Abdelilah-Seyfried, S., Panáková, D.
ID
ZDB-PUB-181212-27
Date
2018
Source
Nature communications   9: 2161 (Journal)
Registered Authors
Abdelilah-Seyfried, Salim, Burger, Alexa, Merks, Anne, Meyer, Alexander, Mosimann, Christian, Panáková, Daniela, Swinarski, Marie
Keywords
none
MeSH Terms
  • Actomyosin/genetics
  • Actomyosin/metabolism*
  • Animals
  • Animals, Genetically Modified
  • Atrial Remodeling/genetics
  • Cell Polarity/genetics
  • Cell Polarity/physiology*
  • Embryonic Development/genetics
  • Heart/embryology
  • Mutation
  • Myocardium/cytology
  • Myocardium/metabolism*
  • Signal Transduction*
  • Ventricular Remodeling/genetics
  • Wnt Proteins/genetics
  • Wnt Proteins/metabolism
  • Wnt-5a Protein/genetics
  • Wnt-5a Protein/metabolism
  • Zebrafish
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/metabolism*
(all 21)
PubMed
29867082 Full text @ Nat. Commun.
Abstract
Development of a multiple-chambered heart from the linear heart tube is inherently linked to cardiac looping. Although many molecular factors regulating the process of cardiac chamber ballooning have been identified, the cellular mechanisms underlying the chamber formation remain unclear. Here, we demonstrate that cardiac chambers remodel by cell neighbour exchange of cardiomyocytes guided by the planar cell polarity (PCP) pathway triggered by two non-canonical Wnt ligands, Wnt5b and Wnt11. We find that PCP signalling coordinates the localisation of actomyosin activity, and thus the efficiency of cell neighbour exchange. On a tissue-scale, PCP signalling planar-polarises tissue tension by restricting the actomyosin contractility to the apical membranes of outflow tract cells. The tissue-scale polarisation of actomyosin contractility is required for cardiac looping that occurs concurrently with chamber ballooning. Taken together, our data reveal that instructive PCP signals couple cardiac chamber expansion with cardiac looping through the organ-scale polarisation of actomyosin-based tissue tension.
Genes / Markers
Marker Marker Type Name
dvl2GENEdishevelled segment polarity protein 2
fzd7aGENEfrizzled class receptor 7a
prickle1aGENEprickle homolog 1a
tnnt2aGENEtroponin T type 2a (cardiac)
vangl2GENEVANGL planar cell polarity protein 2
wnt11f2GENEwnt 11, family member 2
wnt5bGENEwingless-type MMTV integration site family, member 5b
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Figures
Figure Gallery (2 images)
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Expression
Phenotype
Fish Conditions Stage Phenotype Figure
WT + MO1-dvl2controlLong-pec
WT + MO1-dvl2controlLong-pec
WT + MO1-dvl2controlLong-pec
WT + MO1-fzd7acontrolLong-pec
WT + MO1-prickle1acontrolLong-pec
WT + MO1-prickle1acontrolLong-pec
WT + MO2-wnt11f2 + MO2-wnt5bcontrolLong-pec
fzd7ae3/e3controlLong-pec
fzd7ae3/e3controlLong-pec
fzd7ae3/e3controlLong-pec
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Mutations / Transgenics
Allele Construct Type Affected Genomic Region
e3
    		Point Mutation
    m209
      		Point Mutation
      md71TgTransgenic Insertion
        s974TgTransgenic Insertion
          ta98
            		Point Mutation
            twu34TgTransgenic Insertion
              tx226
                		Point Mutation
                1 - 7 of 7
                Show
                Human Disease / Model
                No data available
                Sequence Targeting Reagents
                Target Reagent Reagent Type
                dvl2MO1-dvl2MRPHLNO
                fzd7aMO1-fzd7aMRPHLNO
                prickle1aMO1-prickle1aMRPHLNO
                tnnt2aMO1-tnnt2aMRPHLNO
                vangl2MO1-vangl2MRPHLNO
                wnt5bMO2-wnt5bMRPHLNO
                wnt11f2MO2-wnt11f2MRPHLNO
                1 - 7 of 7
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                Fish
                Fish
                fzd7ae3/e3
                fzd7ae3/e3; s974Tg
                vangl2m209/m209
                vangl2m209/m209; s974Tg
                wnt11f2tx226/tx226; wnt5bta98/ta98
                wnt11f2tx226/tx226; wnt5bta98/ta98; s974Tg
                WT
                WT + MO1-dvl2
                WT + MO1-fzd7a
                WT + MO1-prickle1a
                1 - 10 of 11
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                Antibodies
                Orthology
                No data available
                Engineered Foreign Genes
                Marker Marker Type Name
                EGFPEFGEGFP
                GFPEFGGFP
                VenusEFGVenus
                1 - 3 of 3
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                Mapping
                No data available
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                Citation
                Merks, A.M., Swinarski, M., Meyer, A.M., Müller, N.V., Özcan, I., Donat, S., Burger, A., Gilbert, S., Mosimann, C., Abdelilah-Seyfried, S., Panáková, D. (2018) Planar cell polarity signalling coordinates heart tube remodelling through tissue-scale polarisation of actomyosin activity. Nature communications. 9:2161.