PUBLICATION

Transcriptomic Analysis for Differentially Expressed Genes in Ovarian Follicle Activation in the Zebrafish

Authors
Zhu, B., Pardeshi, L., Chen, Y., Ge, W.
ID
ZDB-PUB-181027-9
Date
2018
Source
Frontiers in endocrinology   9: 593 (Journal)
Registered Authors
Ge, Wei, Zhu, Bo
Keywords
RNA-seq, follicles, folliculogenesis, puberty, transcriptomics, zebrafish
Datasets
GEO:GSE99308
MeSH Terms
none
PubMed
30364302 Full text @ Front Endocrinol (Lausanne)
Abstract
In teleosts, the onset of puberty in females is marked by the appearance of the first wave of pre-vitellogenic (PV) follicles from the pool of primary growth (PG) follicles (follicle activation) in the ovary during sexual maturation. To understand the mechanisms underlying follicle activation and therefore puberty onset, we undertook this transcriptomic study to investigate gene expression profiles in the event. Our analysis revealed a total of 2,027 up-regulated and 859 down-regulated genes during the PG-PV transition. Gene Ontology (GO) analysis showed that in addition to basic cellular functions such as gene transcription, cell differentiation, and cell migration, other biological processes such as steroidogenesis, cell signaling and angiogenesis were also enriched in up-regulated genes; by comparison, some processes were down-regulated including piRNA metabolism, gene silencing and proteolysis. Further Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis identified a variety of signaling pathways that might play pivotal roles in PG-PV transition, including MAPK, TGF-β, Hedgehog, FoxO, VEGF, Jak-STAT, and phosphatidylinositol signaling pathways. Other pathways of particular interest included endocytosis and glycosaminoglycan biosynthesis. We also analyzed expression changes of genes expressed in different compartments viz. oocytes and follicle cells. Interestingly, most oocyte-specific genes remained unchanged in expression during follicle activation whereas a great number of genes specifically expressed in the follicle cells showed significant changes in expression. Overall, this study reported a comprehensive analysis for genes, biological processes and pathways involved in follicle activation, which also marks female puberty onset in the zebrafish when occurring for the first time in sexual maturation.
Genes / Markers
Marker Marker Type Name
amhGENEanti-Mullerian hormone
cyp19a1aGENEcytochrome P450, family 19, subfamily A, polypeptide 1a
fshrGENEfollicle stimulating hormone receptor
gadd45gaGENEgrowth arrest and DNA-damage-inducible, gamma a
inhaGENEinhibin subunit alpha
inhbaaGENEinhibin subunit beta Aa
lplaGENElipoprotein lipase a
marchf4bGENEmembrane associated ring-CH-type finger 4b
notch3GENEnotch receptor 3
ube2ql1GENEubiquitin conjugating enzyme E2 QL1
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