PUBLICATION
Identification and characterization of two zebrafish Twik related potassium channels, Kcnk2a and Kcnk2b
- Authors
- Nasr, N., Faucherre, A., Borsotto, M., Heurteaux, C., Mazella, J., Jopling, C., Moha Ou Maati, H.
- ID
- ZDB-PUB-181020-8
- Date
- 2018
- Source
- Scientific Reports 8: 15311 (Journal)
- Registered Authors
- Faucherre, Adele, Jopling, Chris
- Keywords
- none
- MeSH Terms
-
- Animals
- Antidepressive Agents, Second-Generation/pharmacology
- Cells, Cultured
- Fluoxetine/pharmacology
- HEK293 Cells
- Humans
- Ion Channel Gating/drug effects
- Ion Channel Gating/genetics
- Ion Channel Gating/physiology*
- Membrane Potentials/drug effects
- Membrane Potentials/genetics
- Membrane Potentials/physiology
- Neuroprotective Agents/pharmacology
- Peptides/pharmacology
- Potassium Channels, Tandem Pore Domain/genetics
- Potassium Channels, Tandem Pore Domain/metabolism*
- Potassium Channels, Tandem Pore Domain/physiology
- Protein Isoforms/genetics
- Protein Isoforms/metabolism*
- Protein Isoforms/physiology
- Riluzole/pharmacology
- Zebrafish/genetics
- Zebrafish/metabolism*
- Zebrafish Proteins/genetics
- Zebrafish Proteins/metabolism*
- Zebrafish Proteins/physiology
- PubMed
- 30333618 Full text @ Sci. Rep.
Citation
Nasr, N., Faucherre, A., Borsotto, M., Heurteaux, C., Mazella, J., Jopling, C., Moha Ou Maati, H. (2018) Identification and characterization of two zebrafish Twik related potassium channels, Kcnk2a and Kcnk2b. Scientific Reports. 8:15311.
Abstract
KCNK2 is a 2 pore domain potassium channel involved in maintaining cellular membrane resting potentials. Although KCNK2 is regarded as a mechanosensitive ion channel, it can also be gated chemically. Previous research indicates that KCNK2 expression is particularly enriched in neuronal and cardiac tissues. In this respect, KCNK2 plays an important role in neuroprotection and has also been linked to cardiac arrhythmias. KCNK2 has subsequently become an attractive pharmacologic target for developing preventative/curative strategies for neuro/cardio pathophysiological conditions. Zebrafish represent an important in vivo model for rapidly analysing pharmacological compounds. We therefore sought to identify and characterise zebrafish kcnk2 to allow this model system to be incorporated into therapeutic research. Our data indicates that zebrafish possess two kcnk2 orthologs, kcnk2a and kcnk2b. Electrophysiological analysis of both zebrafish Kcnk2 orthologs shows that, like their human counterparts, they are activated by different physiological stimuli such as mechanical stretch, polyunsaturated fatty acids and intracellular acidification. Furthermore, both zebrafish Kcnk2 channels are inhibited by the human KCNK2 inhibitory peptide spadin. Taken together, our results demonstrate that both Kcnk2a and Kcnk2b share similar biophysiological and pharmacological properties to human KCNK2 and indicate that the zebrafish will be a useful model for developing KCNK2 targeting strategies.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping