PUBLICATION
Evaluation of the estrogen receptor alpha as a possible target of bifenthrin effects in the estrogenic and dopaminergic signaling pathways in zebrafish embryos
- Authors
- Bertotto, L.B., Dasgupta, S., Vliet, S., Dudley, S., Gan, J., Volz, D.C., Schlenk, D.
- ID
- ZDB-PUB-181020-19
- Date
- 2018
- Source
- The Science of the total environment 651: 2424-2431 (Journal)
- Registered Authors
- Keywords
- Aquatic toxicology, Developmental toxicology, ERĪ± knockdown, Endocrine disruption, Pyrethroid pesticides
- MeSH Terms
-
- Animals
- Dopamine/physiology*
- Estrogen Receptor alpha/genetics*
- Estrogen Receptor alpha/metabolism
- Estrogens/physiology*
- Insecticides/adverse effects*
- Pyrethrins/adverse effects*
- Signal Transduction/drug effects*
- Zebrafish/physiology*
- Zebrafish Proteins/genetics*
- Zebrafish Proteins/metabolism
- PubMed
- 30336432 Full text @ Sci. Total Environ.
Citation
Bertotto, L.B., Dasgupta, S., Vliet, S., Dudley, S., Gan, J., Volz, D.C., Schlenk, D. (2018) Evaluation of the estrogen receptor alpha as a possible target of bifenthrin effects in the estrogenic and dopaminergic signaling pathways in zebrafish embryos. The Science of the total environment. 651:2424-2431.
Abstract
Bifenthrin (BF) is a pyrethroid insecticide widely used in urban and agricultural applications. Previous studies in embryos of zebrafish have shown that BF can affect estradiol biosynthesis and the dopaminergic system. To examine the role of the estrogen receptor (ER) in the endocrine effects of BF, embryos were exposed for 96 h to a mixture of 0.15 and 1.5 μg/L BF and an ER agonist (17α-ethynylestradiol - EE2) at 0.09 μg/L. Transcripts related to estrogenic (vitellogenin VTG) and dopaminergic (tyrosine hydroxylase (TH), dopamine receptor 1 (DR1), monoamine oxidase (MAO), and catechol-O-methyltransferase b (COMTb)) signaling pathways were investigated by qRT-PCR. Dopamine (DA) and its metabolites (homovanillic acid (HVA) and 3,4-dihydroxyphenylacetic acid (DOPAC)) were also measured. There was a significant increase in VTG, DR1, MAO and COMTb mRNA levels and HVA-DA ratios within all zebrafish embryos exposed to EE2, including EE2 alone, 0.15 μg/L BF + EE2 and 1.5 μg/L BF + EE2. A significant decrease in homogenate concentrations of DA was observed within all zebrafish embryos exposed to EE2, which included EE2 alone, 0.15 μg/L BF + EE2 and 1.5 μg/L BF + EE2. Co-exposure of BF with EE2 failed to diminish estrogenic or dopaminergic signaling in embryos. Additionally, embryos with diminished ERα expression by morpholino injection were exposed to 0.15 μg/L BF, 1.5 μg/L BF and 0.09 μg/L EE2, with subsequent gene expression measurements. ERα knockdown did not prevent the effects of BF, indicating ERα may have a limited role in the estrogenic and dopaminergic effects caused by BF in zebrafish embryos.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping