PUBLICATION
Spatiotemporal expression of the cocaine- and amphetamine-regulated transcript-like (cart-like) gene during zebrafish embryogenesis
- Authors
- Kawahara, A., Morita, H., Yanagi, K., Suzuki, H., Mori, T., Ohga, R., Taimatsu, K.
- ID
- ZDB-PUB-180821-11
- Date
- 2018
- Source
- Gene expression patterns : GEP 30: 1-6 (Journal)
- Registered Authors
- Kawahara, Atsuo, Morita, Hitoshi, Suzuki, Hiroaki
- Keywords
- Cart-like, Cart1, Cart2, Rohon-Beard neuron, Trigeminal ganglia, Zebrafish
- MeSH Terms
-
- Amino Acid Sequence
- Animals
- Blastula/cytology
- Blastula/metabolism
- Embryo, Nonmammalian/cytology
- Embryo, Nonmammalian/metabolism*
- Embryonic Development
- Gastrula/cytology
- Gastrula/metabolism
- Gene Expression Regulation, Developmental*
- Nerve Tissue Proteins/genetics
- Nerve Tissue Proteins/metabolism*
- Neurogenesis
- Phylogeny
- Sequence Homology
- Spatio-Temporal Analysis
- Zebrafish/embryology*
- Zebrafish/genetics*
- Zebrafish/metabolism
- Zebrafish Proteins/genetics
- Zebrafish Proteins/metabolism*
- PubMed
- 30125742 Full text @ Gene Expr. Patterns
Citation
Kawahara, A., Morita, H., Yanagi, K., Suzuki, H., Mori, T., Ohga, R., Taimatsu, K. (2018) Spatiotemporal expression of the cocaine- and amphetamine-regulated transcript-like (cart-like) gene during zebrafish embryogenesis. Gene expression patterns : GEP. 30:1-6.
Abstract
The cocaine- and amphetamine-regulated transcript (CART) genes are involved in the neural regulation of energy homeostasis; however, their developmental expressions and functions are not fully understood in vertebrates. We have identified a novel zebrafish cart-like gene that encodes a protein of 105 amino acids possessing sequence similarity to zebrafish and mammalian CART proteins. RT-PCR analysis revealed that the cart-like transcripts were maternally supplied and gradually decreased during the cleavage, blastula and gastrula stages; then, transcripts subsequently reaccumulated at the segmentation, pharyngula and hatching stages. Based on a whole-mount in situ hybridization analysis using an antisense cart-like RNA probe, we found that the cart-like transcript was predominantly expressed in both the Rohon-Beard neurons and trigeminal ganglia, suggesting the involvement of the cart-like gene in zebrafish neural development.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping