PUBLICATION

Spatio-temporal regulation of concurrent developmental processes by generic signaling downstream of chemokine receptors

Authors
Malhotra, D., Shin, J., Solnica-Krezel, L., Raz, E.
ID
ZDB-PUB-180607-10
Date
2018
Source
eLIFE   7: (Journal)
Registered Authors
Malhotra, Divyanshu, Raz, Erez, Shin, Jimann, Solnica-Krezel, Lilianna
Keywords
chemokine, chemokine signalling, developmental biology, embryo development, immunology, inflammation, pattern formation, signalling bias, stem cells, zebrafish
MeSH Terms
  • Animals
  • Animals, Genetically Modified
  • Cell Movement/genetics
  • Embryo, Nonmammalian/cytology
  • Embryo, Nonmammalian/embryology
  • Embryo, Nonmammalian/metabolism
  • Gene Expression Profiling
  • Gene Expression Regulation, Developmental*
  • Receptors, CCR/genetics
  • Receptors, CCR/metabolism
  • Receptors, CCR7/genetics
  • Receptors, CCR7/metabolism
  • Receptors, CXCR4/genetics
  • Receptors, CXCR4/metabolism
  • Receptors, Chemokine/genetics*
  • Receptors, Chemokine/metabolism
  • Signal Transduction/genetics*
  • Zebrafish/embryology
  • Zebrafish/genetics*
  • Zebrafish/metabolism
  • Zebrafish Proteins/genetics*
  • Zebrafish Proteins/metabolism
(all 22)
PubMed
29873633 Full text @ Elife
Abstract
Chemokines are secreted proteins that regulate a range of processes in eukaryotic organisms. Interestingly, different chemokine receptors control distinct biological processes, and the same receptor can direct different cellular responses, but the basis for this phenomenon is not known. To understand this property of chemokine signaling, we examined the function of the chemokine receptors Cxcr4a, Cxcr4b, Ccr7, Ccr9 in the context of diverse processes in embryonic development in zebrafish. Our results reveal that the specific response to chemokine signaling is dictated by cell-type-specific chemokine receptor signal interpretation modules (CRIM) rather than by chemokine-receptor-specific signals. Thus, a generic signal provided by different receptors leads to discrete responses that depend on the specific identity of the cell that receives the signal. We present the implications of employing generic signals in different contexts such as gastrulation, axis specification and single-cell migration.
Genes / Markers
Marker Marker Type Name
ccr7GENEchemokine (C-C motif) receptor 7
ccr9aGENEchemokine (C-C motif) receptor 9a
cxcl12aGENEchemokine (C-X-C motif) ligand 12a (stromal cell-derived factor 1)
cxcl12bGENEchemokine (C-X-C motif) ligand 12b (stromal cell-derived factor 1)
cxcr4aGENEchemokine (C-X-C motif) receptor 4a
cxcr4bGENEchemokine (C-X-C motif), receptor 4b
nanos3GENEnanos homolog 3
plpp1aGENEphospholipid phosphatase 1a
plpp3GENEphospholipid phosphatase 3
1 - 9 of 9
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Figures
Figure Gallery (10 images)
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Expression
Phenotype
Fish Conditions Stage Phenotype Figure
ha01Tg + MO1-cxcr4acontrol75%-epiboly
ha01Tg + MO1-cxcr4a + MO5-cxcl12bcontrol75%-epiboly
1 - 2 of 2
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Mutations / Transgenics
Allele Construct Type Affected Genomic Region
ha01TgTransgenic Insertion
    ml1TgTransgenic Insertion
      stl7
        		Small Deletion
        t26035
          		Point Mutation
          1 - 4 of 4
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          Human Disease / Model
          No data available
          Sequence Targeting Reagents
          Fish
          Antibodies
          Orthology
          Engineered Foreign Genes
          Marker Marker Type Name
          EGFPEFGEGFP
          GFPEFGGFP
          1 - 2 of 2
          Show
          Mapping
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          Citation
          Malhotra, D., Shin, J., Solnica-Krezel, L., Raz, E. (2018) Spatio-temporal regulation of concurrent developmental processes by generic signaling downstream of chemokine receptors. eLIFE. 7.