PUBLICATION

Hippo signaling determines the number of venous pole cells that originate from the anterior lateral plate mesoderm in zebrafish

Authors
Fukui, H., Miyazaki, T., Chow, R.W., Ishikawa, H., Nakajima, H., Vermot, J., Mochizuki, N.
ID
ZDB-PUB-180530-37
Date
2018
Source
eLIFE   7: (Journal)
Registered Authors
Fukui, Hajime, Mochizuki, Naoki, Nakajima, Hiroyuki, Vermot, Julien
Keywords
developmental biology, stem cells, zebrafish
MeSH Terms
  • Animals
  • Basic Helix-Loop-Helix Transcription Factors/genetics
  • Basic Helix-Loop-Helix Transcription Factors/metabolism
  • Bone Morphogenetic Proteins/genetics
  • Bone Morphogenetic Proteins/metabolism
  • Cell Count
  • Cell Differentiation
  • Cell Proliferation
  • Embryo, Nonmammalian
  • Gene Expression Regulation, Developmental
  • Heart Atria/cytology
  • Heart Atria/growth & development
  • Heart Atria/metabolism*
  • Mesoderm/cytology
  • Mesoderm/growth & development
  • Mesoderm/metabolism*
  • Myocardium/cytology
  • Myocardium/metabolism
  • Myocytes, Cardiac/cytology
  • Myocytes, Cardiac/metabolism*
  • Organogenesis/genetics
  • Protein Serine-Threonine Kinases/genetics
  • Protein Serine-Threonine Kinases/metabolism*
  • Signal Transduction/genetics*
  • Tumor Suppressor Proteins/genetics
  • Tumor Suppressor Proteins/metabolism
  • Zebrafish
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/metabolism*
(all 29)
PubMed
29809141 Full text @ Elife
Abstract
The differentiation of the lateral plate mesoderm cells into heart field cells constitutes a critical step in the development of cardiac tissue and the genesis of functional cardiomyocytes. Hippo signaling controls cardiomyocyte proliferation, but the role of Hippo signaling during early cardiogenesis remains unclear. Here, we show that Hippo signaling regulates atrial cell number by specifying the developmental potential of cells within the anterior lateral plate mesoderm (ALPM), which are incorporated into the venous pole of the heart tube and ultimately into the atrium of the heart. We demonstrate that Hippo signaling acts through large tumor suppressor kinase 1/2 to modulate BMP signaling and the expression of hand2, a key transcription factor that is involved in the differentiation of atrial cardiomyocytes. Collectively, these results demonstrate that Hippo signaling defines venous pole cardiomyocyte number by modulating both the number and the identity of the ALPM cells that will populate the atrium of the heart.
Genes / Markers
Marker Marker Type Name
ajubaGENEajuba LIM protein
bmp2bGENEbone morphogenetic protein 2b
bmp4GENEbone morphogenetic protein 4
etsrpGENEETS1-related protein
gata4GENEGATA binding protein 4
hand2GENEheart and neural crest derivatives expressed 2
hoxb5bGENEhomeobox B5b
isl1aGENEISL LIM homeobox 1a
lats1GENElarge tumor suppressor kinase 1
lats2GENElarge tumor suppressor kinase 2
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Figures
Figure Gallery (17 images) / 2
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Expression
Phenotype
Fish Conditions Stage Phenotype Figure
AB + MO2-lats1 + MO2-lats2controlPrim-5
lats1ncv107/+; lats2ncv108/+control10-13 somites
lats1ncv107/+; lats2ncv108/ncv108; mw29Tg; ncv11TgcontrolPrim-5
lats1ncv107/+; lats2ncv108/ncv108; ncv11Tg; ncv45TgcontrolProtruding-mouth
lats1ncv107/+; lats2ncv108/ncv108; ncv11Tg; ncv47TgcontrolPrim-5
lats1ncv107/ncv107; lats2ncv108/ncv108control10-13 somites
lats1ncv107/ncv107; lats2ncv108/ncv108control10-13 somites
lats1ncv107/ncv107; lats2ncv108/ncv108control10-13 somites
lats1ncv107/ncv107; lats2ncv108/ncv108control10-13 somites
lats1ncv107/ncv107; lats2ncv108/ncv108control10-13 somites
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Mutations / Transgenics
Allele Construct Type Affected Genomic Region
mw29TgTransgenic Insertion
    ncv11TgTransgenic Insertion
      ncv12TgTransgenic Insertion
        ncv45TgTransgenic Insertion
          ncv46TgTransgenic Insertion
            ncv47TgTransgenic Insertion
              ncv65TgTransgenic Insertion
                ncv107
                  		Small Deletion
                  ncv108
                    		Small Deletion
                    ncv114
                      		Small Deletion
                      1 - 10 of 14
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                      Human Disease / Model
                      Sequence Targeting Reagents
                      Fish
                      Antibodies
                      Orthology
                      Engineered Foreign Genes
                      Marker Marker Type Name
                      EGFPEFGEGFP
                      EosEFGEos
                      GAL4EFGGAL4
                      GFPEFGGFP
                      mCherryEFGmCherry
                      RFPEFGRFP
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                      Mapping
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                      Citation
                      Fukui, H., Miyazaki, T., Chow, R.W., Ishikawa, H., Nakajima, H., Vermot, J., Mochizuki, N. (2018) Hippo signaling determines the number of venous pole cells that originate from the anterior lateral plate mesoderm in zebrafish. eLIFE. 7.