PUBLICATION
Metabolic and bone effects of high-fat diet in adult zebrafish
- Authors
- Carnovali, M., Luzi, L., Terruzzi, I., Banfi, G., Mariotti, M.
- ID
- ZDB-PUB-171224-3
- Date
- 2017
- Source
- Endocrine 61(2): 317-326 (Journal)
- Registered Authors
- Keywords
- Bone, Insulin, Obesity, Osteoporosis, Zebrafish
- MeSH Terms
-
- Age Factors
- Animals
- Blood Glucose/metabolism
- Bone Density
- Bone and Bones/pathology
- Bone and Bones/physiology*
- Diet, High-Fat/adverse effects*
- Disease Models, Animal
- Energy Metabolism/physiology*
- Obesity/etiology
- Obesity/metabolism*
- Obesity/pathology*
- Zebrafish*
- PubMed
- 29274064 Full text @ Endocrine
Citation
Carnovali, M., Luzi, L., Terruzzi, I., Banfi, G., Mariotti, M. (2017) Metabolic and bone effects of high-fat diet in adult zebrafish. Endocrine. 61(2):317-326.
Abstract
An increase of visceral fat affects human bone health causing fragility, mechanical strength reduction, and increased propensity to fractures because of impaired bone matrix microstructure and aberrant bone cell function. Adult Danio rerio (zebrafish) represents a powerful model to study both metabolic diseases and bone metabolism. The aim of this study was to generate an obese adult zebrafish by high-fat diet and evaluate metabolic and bone tissue effects. Fish blood glucose and insulin levels were found to be altered in high-fat diet fish revealing a failure in β-cells insulin production. Blood analysis of adipokines revealed significant alterations in adiponectin and leptin levels that are common in human and other obesity animal models. Advanced glycation end products (AGEs), derived from hyperglycemia condition, were found to be altered too. All these alterations were associated with an impaired bone metabolism. The scales of high-fat diet fish shown bone resorption lacunae associated with an intense osteoclastic tartrate-resistant acid phosphatase (TRAP) activity, whereas alkaline phosphatase (ALP) decreased. These data suggest that an imbalance of fat metabolism alters energy metabolism generating an osteoporosis-like phenotype in adult zebrafish scales. The zebrafish obesity model can contribute to elucidate in vivo the molecular mechanisms of metabolic changes in human obese patients.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping