PUBLICATION
Establishment of a three-step method to evaluate effects of chemicals on development of zebrafish embryo/larvae
- Authors
- Li, G., Ye, H., Su, G., Han, Z., Xie, C., Zhou, B., Letcher, R.J., Giesy, J.P., Yu, H., Liu, C.
- ID
- ZDB-PUB-170808-5
- Date
- 2017
- Source
- Chemosphere 186: 209-217 (Journal)
- Registered Authors
- Zhou, BingSheng
- Keywords
- CdCl(2), Chemical screening, Developmental toxicity, FTOH, Morphology, TDCIPP
- MeSH Terms
-
- Animals
- Embryo, Nonmammalian/drug effects*
- Embryonic Development/drug effects*
- Hexanols
- Larva/drug effects
- Organophosphates
- Toxicity Tests/methods*
- Water Pollutants, Chemical/toxicity*
- Zebrafish/embryology
- Zebrafish/growth & development*
- PubMed
- 28780448 Full text @ Chemosphere
Citation
Li, G., Ye, H., Su, G., Han, Z., Xie, C., Zhou, B., Letcher, R.J., Giesy, J.P., Yu, H., Liu, C. (2017) Establishment of a three-step method to evaluate effects of chemicals on development of zebrafish embryo/larvae. Chemosphere. 186:209-217.
Abstract
Core endpoints in zebrafish embryos are crucial indicators in screening harmful effects of chemicals. In this study, we established a three-step process to more quantitatively and less-subjective determine effects of chemicals on phenotypes of developing zebrafish embryos. Embryos were exposed to each of two concentrations of the representative chemicals cadmium chloride (CdCl2), tris(1,3-dichloro-2-propyl) phosphate (TDCIPP) or 1H, 1H, 2H, 2H-nonafluoro-1-hexanol (4:2 FTOH) from 0.75 h post-fertilization (hpf) to 96 hpf. After exposure, larvae were imaged by use of a three-step method to describe morphology. Seven points were selected, which resulted in acquisition of 21 lines and 105 angles from images of larvae. Exposure to TDCIPP (0.1 or 0.2 mg/L), CdCl2 (1 or 4 mg/L) or 4:2 FTOH (0.3 or 1 mg/L) significantly changed lengths of some lines and magnitudes of some angles, that resulted in differential scoring of points. Points were then prioritized and directions, distances and trajectories of movement were further described and standard reference values were developed. Movement of the point describing the mouth during embryonic development was found to be a sensitive parameter for assessment of adverse effects of chemicals. The present study provides a new strategy to characterize phenotypes of development of zebrafish embryo/larva following exposure to environmental toxins.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping