PUBLICATION

Microarray-based bioinformatics analysis of the combined effects of SiNPs and PbAc on cardiovascular system in zebrafish

Authors
Hu, H., Zhang, Y., Shi, Y., Feng, L., Duan, J., Sun, Z.
ID
ZDB-PUB-170707-10
Date
2017
Source
Chemosphere   184: 1298-1309 (Journal)
Registered Authors
Keywords
Bioinformatics analysis, Cardiovascular system, Combined toxicity, Microarray analysis, PbAc, SiNPs
MeSH Terms
  • Animals
  • Cardiovascular System/drug effects*
  • Cardiovascular System/pathology
  • Computational Biology
  • Humans
  • MicroRNAs/genetics
  • Nanoparticles/toxicity*
  • No-Observed-Adverse-Effect Level
  • Organometallic Compounds/toxicity*
  • Phenotype
  • RNA, Messenger/metabolism
  • Silicon/toxicity*
  • Toxicity Tests/methods*
  • Water Pollutants, Chemical/toxicity*
  • Zebrafish/metabolism
  • Zebrafish/physiology*
(all 16)
PubMed
28679150 Full text @ Chemosphere
Abstract
With rapid development of nanotechnology and growing environmental pollution, the combined toxic effects of SiNPs and pollutants of heavy metals like lead have received global attentions. The aim of this study was to explore the cardiovascular effects of the co-exposure of SiNPs and lead acetate (PbAc) in zebrafish using microarray and bioinformatics analysis. Although there was no other obvious cardiovascular malformation except bleeding phenotype, bradycardia, angiogenesis inhibition and declined cardiac output in zebrafish co-exposed of SiNPs and PbAc at NOAEL level, significant changes were observed in mRNA and microRNA (miRNA) expression patterns. STC-GO analysis indicated that the co-exposure might have more toxic effects on cardiovascular system than that exposure alone. Key differentially expressed genes were discerned out based on the Dynamic-gene-network, including stxbp1a, ndfip2, celf4 and gsk3b. Furthermore, several miRNAs obtained from the miRNA-Gene-Network might play crucial roles in cardiovascular disease, such as dre-miR-93, dre-miR-34a, dre-miR-181c, dre-miR-7145, dre-miR-730, dre-miR-129-5p, dre-miR-19d, dre-miR-218b, dre-miR-221. Besides, the analysis of miRNA-pathway-network indicated that the zebrafish were stimulated by the co-exposure of SiNPs and PbAc, which might cause the disturbance of calcium homeostasis and endoplasmic reticulum stress. As a result, cardiac muscle contraction might be deteriorated. In general, our data provide abundant fundamental research clues to the combined toxicity of environmental pollutants and further in-depth verifications are needed.
Genes / Markers
Marker Marker Type Name
celf4GENECUGBP, Elav-like family member 4
dre-mir-221MIRNAGmicroRNA 221
gsk3baGENEglycogen synthase kinase 3 beta, genome duplicate a
mir181cMIRNAGmicroRNA 181c
mir19dMIRNAGmicroRNA 19d
mir218bMIRNAGmicroRNA 218b
mir34aMIRNAGmicroRNA 34a
mir7145MIRNAGmicroRNA 7145
mir730MIRNAGmicroRNA 730
mir93MIRNAGmicroRNA 93
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Figures
No images available
Expression
Phenotype
No data available
Mutations / Transgenics
Allele Construct Type Affected Genomic Region
b4
    		Insertion
    y1TgTransgenic Insertion
      1 - 2 of 2
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      Human Disease / Model
      No data available
      Sequence Targeting Reagents
      No data available
      Fish
      No data available
      Antibodies
      No data available
      Orthology
      No data available
      Engineered Foreign Genes
      Marker Marker Type Name
      EGFPEFGEGFP
      1 - 1 of 1
      Show
      Mapping
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      Citation
      Hu, H., Zhang, Y., Shi, Y., Feng, L., Duan, J., Sun, Z. (2017) Microarray-based bioinformatics analysis of the combined effects of SiNPs and PbAc on cardiovascular system in zebrafish. Chemosphere. 184:1298-1309.