PUBLICATION

Hesperidin Protects against Acute Alcoholic Injury through Improving Lipid Metabolism and Cell Damage in Zebrafish Larvae

Authors
Zhou, Z., Zhong, W., Lin, H., Huang, P., Ma, N., Zhang, Y., Zhou, C., Lai, Y., Huang, S., Huang, S., Gao, L., Lv, Z.
ID
ZDB-PUB-170610-8
Date
2017
Source
Evidence-based complementary and alternative medicine : eCAM   2017: 7282653 (Journal)
Registered Authors
Ma, Ning, Zhang, Yuqing
Keywords
none
MeSH Terms
none
PubMed
28596796 Full text @ Evid. Based Complement. Alternat. Med.
Abstract
Alcoholic liver disease (ALD) is a series of abnormalities of liver function, including alcoholic steatosis, steatohepatitis, and cirrhosis. Hesperidin, the major constituent of flavanone in grapefruit, is proved to play a role in antioxidation, anti-inflammation, and reducing multiple organs damage in various animal experiments. However, the underlying mechanism of resistance to alcoholic liver injury is still unclear. Thus, we aimed to investigate the protective effects of hesperidin against ALD and its molecular mechanism in this study. We established an ALD zebrafish larvae model induced by 350 mM ethanol for 32 hours, using wild-type and transgenic line with liver-specific eGFP expression Tg (lfabp10α:eGFP) zebrafish larvae (4 dpf). The results revealed that hesperidin dramatically reduced the hepatic morphological damage and the expressions of alcohol and lipid metabolism related genes, including cyp2y3, cyp3a65, hmgcra, hmgcrb, fasn, and fads2 compared with ALD model. Moreover, the findings demonstrated that hesperidin alleviated hepatic damage as well, which is reflected by the expressions of endoplasmic reticulum stress and DNA damage related genes (chop, gadd45αa, and edem1). In conclusion, this study revealed that hesperidin can inhibit alcoholic damage to liver of zebrafish larvae by reducing endoplasmic reticulum stress and DNA damage, regulating alcohol and lipid metabolism.
Genes / Markers
Marker Marker Type Name
cyp2y3GENEcytochrome P450, family 2, subfamily Y, polypeptide 3
cyp3a65GENEcytochrome P450, family 3, subfamily A, polypeptide 65
ddit3GENEDNA-damage-inducible transcript 3
edem1GENEER degradation enhancer, mannosidase alpha-like 1
fabp10aGENEfatty acid binding protein 10a, liver basic
fads2GENEfatty acid desaturase 2
fasnGENEfatty acid synthase
gadd45aaGENEgrowth arrest and DNA-damage-inducible, alpha, a
hmgcraGENE3-hydroxy-3-methylglutaryl-CoA reductase a
hmgcrbGENE3-hydroxy-3-methylglutaryl-CoA reductase b
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Figures
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Expression
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay Figure
cyp2y3ABstandard conditionsDay 5RTPCR
cyp2y3ABchemical treatment by environment: ethanolDay 5RTPCR
cyp2y3ABchemical treatment by environment: hesperidin, chemical treatment by environment: ethanolDay 5RTPCR
cyp3a65ABchemical treatment by environment: ethanolDay 5RTPCR
cyp3a65ABchemical treatment by environment: hesperidin, chemical treatment by environment: ethanolDay 5RTPCR
cyp3a65ABstandard conditionsDay 5RTPCR
ddit3ABstandard conditionsDay 5RTPCR
ddit3ABchemical treatment by environment: hesperidin, chemical treatment by environment: ethanolDay 5RTPCR
ddit3ABchemical treatment by environment: ethanolDay 5RTPCR
edem1ABstandard conditionsDay 5RTPCR
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Phenotype
Fish Conditions Stage Phenotype Figure
ABchemical treatment by environment: hesperidin, chemical treatment by environment: ethanolDay 5
ABchemical treatment by environment: hesperidin, chemical treatment by environment: ethanolDay 5
ABchemical treatment by environment: hesperidin, chemical treatment by environment: ethanolDay 5
ABchemical treatment by environment: hesperidin, chemical treatment by environment: ethanolDay 5
ABchemical treatment by environment: hesperidin, chemical treatment by environment: ethanolDay 5
ABchemical treatment by environment: hesperidin, chemical treatment by environment: ethanolDay 5
ABchemical treatment by environment: hesperidin, chemical treatment by environment: ethanolDay 5
ABchemical treatment by environment: hesperidin, chemical treatment by environment: ethanolDay 5
ABchemical treatment by environment: hesperidin, chemical treatment by environment: ethanolDay 5
ABchemical treatment by environment: hesperidin, chemical treatment by environment: ethanolDay 5
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Mutations / Transgenics
Allele Construct Type Affected Genomic Region
zf1071TgTransgenic Insertion
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    Human Disease / Model
    Human Disease Fish Conditions Evidence
    steatotic liver diseaseABchemical treatment by environment: ethanolTAS
    1 - 1 of 1
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    Sequence Targeting Reagents
    No data available
    Fish
    Antibodies
    Orthology
    Engineered Foreign Genes
    Marker Marker Type Name
    EGFPEFGEGFP
    1 - 1 of 1
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    Mapping
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    Citation
    Zhou, Z., Zhong, W., Lin, H., Huang, P., Ma, N., Zhang, Y., Zhou, C., Lai, Y., Huang, S., Huang, S., Gao, L., Lv, Z. (2017) Hesperidin Protects against Acute Alcoholic Injury through Improving Lipid Metabolism and Cell Damage in Zebrafish Larvae. Evidence-based complementary and alternative medicine : eCAM. 2017:7282653.