PUBLICATION
Autophagosome-lysosome fusion triggers a lysosomal response mediated by TLR9 and controlled by OCRL
- Authors
- De Leo, M.G., Staiano, L., Vicinanza, M., Luciani, A., Carissimo, A., Mutarelli, M., Di Campli, A., Polishchuk, E., Di Tullio, G., Morra, V., Levtchenko, E., Oltrabella, F., Starborg, T., Santoro, M., di Bernardo, D., Devuyst, O., Lowe, M., Medina, D.L., Ballabio, A., De Matteis, M.A.
- ID
- ZDB-PUB-170607-12
- Date
- 2016
- Source
- Nature cell biology 18: 839-50 (Journal)
- Registered Authors
- Lowe, Martin, Oltrabella, Francesca
- Keywords
- Autophagosomes, Autophagy, Lysosomes
- MeSH Terms
-
- Animals
- Autophagosomes/physiology*
- Autophagy/genetics
- Autophagy/physiology*
- Cell Line
- Humans
- Lysosomes/metabolism*
- Mutation/genetics
- Oculocerebrorenal Syndrome/genetics
- Oculocerebrorenal Syndrome/metabolism
- Phosphatidylinositol 4,5-Diphosphate/metabolism
- Phosphatidylinositols/genetics*
- Phosphoric Monoester Hydrolases/genetics*
- Phosphoric Monoester Hydrolases/metabolism
- Toll-Like Receptor 9/genetics*
- Zebrafish
- PubMed
- 27398910 Full text @ Nat. Cell Biol.
Citation
De Leo, M.G., Staiano, L., Vicinanza, M., Luciani, A., Carissimo, A., Mutarelli, M., Di Campli, A., Polishchuk, E., Di Tullio, G., Morra, V., Levtchenko, E., Oltrabella, F., Starborg, T., Santoro, M., di Bernardo, D., Devuyst, O., Lowe, M., Medina, D.L., Ballabio, A., De Matteis, M.A. (2016) Autophagosome-lysosome fusion triggers a lysosomal response mediated by TLR9 and controlled by OCRL. Nature cell biology. 18:839-50.
Abstract
Phosphoinositides (PtdIns) control fundamental cell processes, and inherited defects of PtdIns kinases or phosphatases cause severe human diseases, including Lowe syndrome due to mutations in OCRL, which encodes a PtdIns(4,5)P2 5-phosphatase. Here we unveil a lysosomal response to the arrival of autophagosomal cargo in which OCRL plays a key part. We identify mitochondrial DNA and TLR9 as the cargo and the receptor that triggers and mediates, respectively, this response. This lysosome-cargo response is required to sustain the autophagic flux and involves a local increase in PtdIns(4,5)P2 that is confined in space and time by OCRL. Depleting or inhibiting OCRL leads to an accumulation of lysosomal PtdIns(4,5)P2, an inhibitor of the calcium channel mucolipin-1 that controls autophagosome-lysosome fusion. Hence, autophagosomes accumulate in OCRL-depleted cells and in the kidneys of Lowe syndrome patients. Importantly, boosting the activity of mucolipin-1 with selective agonists restores the autophagic flux in cells from Lowe syndrome patients.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping