PUBLICATION
Development of the larval lymphatic system in the zebrafish
- Authors
- Jung, H.M., Castranova, D., Swift, M.R., Pham, V.N., Galanternik, M.V., Isogai, S., Butler, M.G., Mulligan, T.S., Weinstein, B.M.
- ID
- ZDB-PUB-170517-11
- Date
- 2017
- Source
- Development (Cambridge, England) 144(11): 2070-2081 (Journal)
- Registered Authors
- Keywords
- cardinal lymphatic, collateral cardinal lymphatic, lateral lymphatic, lymphatic, mrc1a, spinal lymphatic, thoracic duct, zebrafish
- MeSH Terms
-
- Animals
- Animals, Genetically Modified
- Gene Expression Regulation, Developmental
- Green Fluorescent Proteins/metabolism
- Larva/growth & development
- Larva/metabolism
- Lymphatic System/growth & development*
- Lymphatic System/metabolism*
- Lymphatic Vessels/metabolism
- Transgenes
- Veins/metabolism
- Zebrafish/genetics
- Zebrafish/growth & development*
- Zebrafish/metabolism*
- Zebrafish Proteins/metabolism
- PubMed
- 28506987 Full text @ Development
Citation
Jung, H.M., Castranova, D., Swift, M.R., Pham, V.N., Galanternik, M.V., Isogai, S., Butler, M.G., Mulligan, T.S., Weinstein, B.M. (2017) Development of the larval lymphatic system in the zebrafish. Development (Cambridge, England). 144(11):2070-2081.
Abstract
The lymphatic vascular system is a hierarchically organized complex network essential for tissue fluid homeostasis, immune trafficking and absorption of dietary fats in the human body. Despite its importance, the assembly of the lymphatic network is still not fully understood. The zebrafish is a powerful model organism that enables study of lymphatic vessel development using high-resolution imaging and sophisticated genetic and experimental manipulation. Although several studies have described early lymphatic development in the fish, lymphatic development at later stages has not been completely elucidated. In this study, we generated a new Tg(mrc1a:egfp)y251 transgenic zebrafish that uses a mannose receptor, C type 1 (mrc1a) promoter to drive strong EGFP expression in lymphatic vessels at all stages of development and in adult zebrafish. We used this line to describe the assembly of the major vessels of the trunk lymphatic vascular network, including the later-developing collateral cardinal, spinal, superficial lateral and superficial intersegmental lymphatics. Our results show that major trunk lymphatic vessels are conserved in the zebrafish, and provide a thorough and complete description of trunk lymphatic vessel assembly.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping