PUBLICATION

The careg element reveals a common regulation of regeneration in the zebrafish myocardium and fin

Authors
Pfefferli, C., Jaźwińska, A.
ID
ZDB-PUB-170504-6
Date
2017
Source
Nature communications   8: 15151 (Journal)
Registered Authors
Jazwinska, Anna
Keywords
Cell lineage, Regeneration
MeSH Terms
  • Activins/metabolism*
  • Animal Fins/growth & development*
  • Animals
  • Connective Tissue Growth Factor/genetics
  • Female
  • Gene Expression Regulation/genetics
  • Genes, Reporter/genetics*
  • Green Fluorescent Proteins/genetics
  • Heart/growth & development*
  • Inhibins/metabolism*
  • Male
  • Myocardium/cytology*
  • Myocytes, Cardiac/cytology
  • Regeneration/genetics*
  • Regeneration/physiology
  • Transforming Growth Factor beta/antagonists & inhibitors
  • Transforming Growth Factor beta/metabolism*
  • Wound Healing/genetics
  • Wound Healing/physiology
  • Zebrafish/genetics
  • Zebrafish Proteins/genetics
(all 21)
PubMed
28466843 Full text @ Nat. Commun.
Abstract
The existence of common mechanisms regulating organ regeneration is an intriguing concept. Here we report on a regulatory element that is transiently activated during heart and fin regeneration in zebrafish. This element contains a ctgfa upstream sequence, called careg, which is induced by TGFβ/Activin-β signalling in the peri-injury zone of the myocardium and the fin mesenchyme. In addition, this reporter demarcates a primordial cardiac layer and intraray osteoblasts. Using genetic fate mapping, we show the regenerative competence of careg-expressing cells. The analysis of the heart reveals that the primordial cardiac layer is incompletely restored after cryoinjury, whereas trabecular and cortical cardiomyocytes contribute to myocardial regrowth. In regenerating fins, the activated mesenchyme of the stump gives rise to the blastema. Our findings provide evidence of a common regenerative programme in cardiomyocytes and mesenchyme that opens the possibility to further explore conserved mechanisms of the cellular plasticity in diverse vertebrate organs.
Genes / Markers
Marker Marker Type Name
caregRRcareg element
ccn2aGENEcellular communication network factor 2a
fgf20aGENEfibroblast growth factor 20a
msx1bGENEmuscle segment homeobox 1b
1 - 4 of 4
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Figures
Figure Gallery (18 images) / 2
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Expression
Phenotype
No data available
Mutations / Transgenics
Allele Construct Type Affected Genomic Region
cz1701TgTransgenic Insertion
    f1TgTransgenic Insertion
      f2TgTransgenic Insertion
        fb2TgTransgenic Insertion
          fri1TgTransgenic Insertion
            fri2TgTransgenic Insertion
              li7TgTransgenic Insertion
                zf620TgTransgenic Insertion
                  zp3
                    		Point Mutation
                    1 - 9 of 9
                    Show
                    Human Disease / Model
                    No data available
                    Sequence Targeting Reagents
                    No data available
                    Fish
                    Fish
                    f2Tg; zf620Tg
                    fri1Tg; zf620Tg
                    WT
                    zf620Tg
                    1 - 4 of 4
                    Show
                    Antibodies
                    Orthology
                    No data available
                    Engineered Foreign Genes
                    Marker Marker Type Name
                    AmCyanEFGAmCyan
                    CreEFGCre
                    DsRed2EFGDsRed2
                    EGFPEFGEGFP
                    GFPEFGGFP
                    mCherryEFGmCherry
                    mKOFP2EFGmKOFP2
                    ZsYellowEFGZsYellow
                    1 - 8 of 8
                    Show
                    Mapping
                    No data available
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                    Citation
                    Pfefferli, C., Jaźwińska, A. (2017) The careg element reveals a common regulation of regeneration in the zebrafish myocardium and fin. Nature communications. 8:15151.