PUBLICATION
Evolution of the genome 3D organization: comparison of fused and segregated globin gene clusters
- Authors
- Kovina, A.P., Petrova, N.V., Gushchanskaya, E.S., Dolgushin, K.V., Gerasimov, E.S., Galitsyna, A.A., Penin, A.A., Flyamer, I.M., Ioudinkova, E.S., Gavrilov, A.A., Vassetzky, Y.S., Ulianov, S.V., Iarovaia, O.V., Razin, S.V.
- ID
- ZDB-PUB-170324-5
- Date
- 2017
- Source
- Molecular Biology and Evolution 34(6): 1492-1504 (Journal)
- Registered Authors
- Keywords
- globin genes, chromatin, 3D organization of the genome, Danio rerio
- Datasets
- GEO:GSE87604, GEO:GSE86548
- MeSH Terms
-
- Animals
- Biological Evolution
- Birds/genetics
- Chromatin/genetics*
- Chromosomes/genetics
- Evolution, Molecular
- Genome
- Globins/genetics*
- Mammals/genetics
- Molecular Conformation*
- Multigene Family/genetics
- Zebrafish/genetics
- alpha-Globins/genetics
- beta-Globins/genetics
- PubMed
- 28333290 Full text @ Mol Bio Evol
Citation
Kovina, A.P., Petrova, N.V., Gushchanskaya, E.S., Dolgushin, K.V., Gerasimov, E.S., Galitsyna, A.A., Penin, A.A., Flyamer, I.M., Ioudinkova, E.S., Gavrilov, A.A., Vassetzky, Y.S., Ulianov, S.V., Iarovaia, O.V., Razin, S.V. (2017) Evolution of the genome 3D organization: comparison of fused and segregated globin gene clusters. Molecular Biology and Evolution. 34(6):1492-1504.
Abstract
The genomes are folded in a complex three-dimensional (3D) structure. Some features of this organization are common for all eukaryotes, but little is known about its evolution. Here we have studied the 3D organization and regulation of zebrafish globin domain and compared its organization and regulation with those of other vertebrate species. In birds and mammals, the alpha- and beta-globin genes are segregated into separate clusters located on different chromosomes and organized into chromatin domains of different types, whereas in cold-blooded vertebrates, including Danio rerio, alpha- and beta-globin genes are organized into common clusters. The major globin gene locus of Danio rerio is of particular interest as it is located in a genomic area that is syntenic in vertebrates and is controlled by a conserved enhancer. We have found that the major globin gene locus of Danio rerio is structurally and functionally segregated into two spatially distinct subloci harboring either adult or embryo-larval globin genes. These subloci demonstrate different organization at the level of chromatin domains and different modes of spatial organization, which appears to be due to selective interaction of the upstream enhancer with the sublocus harboring globin genes of the adult type. These data are discussed in terms of evolution of linear and 3D organization of gene clusters in vertebrates.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping