PUBLICATION
Activity of etv5a and etv5b genes in the hypothalamus of fasted zebrafish is influenced by serotonin
- Authors
- Mechaly, A.S., Richardson, E., Rinkwitz, S.
- ID
- ZDB-PUB-170104-9
- Date
- 2017
- Source
- General and comparative endocrinology 246: 233-240 (Journal)
- Registered Authors
- Mechaly, Alejandro, Rinkwitz, Silke
- Keywords
- Fasting, Fluoxetine, Mood, Serotonin, Zebrafish, obesity, etv5a, etv5b
- MeSH Terms
-
- Animals
- Eating/drug effects
- Eating/physiology
- Fasting/physiology*
- Fish Proteins/genetics*
- Gene Expression Regulation/drug effects*
- Hypothalamus/drug effects
- Hypothalamus/metabolism*
- RNA, Messenger/genetics
- Real-Time Polymerase Chain Reaction
- Receptors, Serotonin/chemistry
- Receptors, Serotonin/genetics
- Reverse Transcriptase Polymerase Chain Reaction
- Serotonin/pharmacology*
- Serotonin Receptor Agonists/pharmacology*
- Zebrafish/growth & development
- Zebrafish/metabolism*
- PubMed
- 28041791 Full text @ Gen. Comp. Endocrinol.
Citation
Mechaly, A.S., Richardson, E., Rinkwitz, S. (2017) Activity of etv5a and etv5b genes in the hypothalamus of fasted zebrafish is influenced by serotonin. General and comparative endocrinology. 246:233-240.
Abstract
Serotonin has been implicated in the inhibition of food intake in vertebrates. However, the mechanisms through which serotonin acts has yet to be elucidated. Recently, ETV5 (ets variant gene 5) has been associated with obesity and food intake control mechanisms in mammals. We have analyzed a putative physiological function of the two etv5 paralogous genes (etv5a and etv5b) in neuronal food intake control in adult zebrafish that have been exposed to different nutritional conditions. A feeding assay was established and fluoxetine, a selective serotonin re-uptake inhibitor (SSRI), was applied. Gene expression changes in the hypothalamus were determined using real-time PCR. Fasting induced an up-regulation of etv5a and etv5b in the hypothalamus, whereas increased serotonin levels in the fasted fish counteracted the increase in expression. To investigate potential mechanisms the expression of further food intake control genes was determined. The results show that an increase of serotonin in fasting fish causes a reduction in the activity of genes stimulating food intake. This is in line with a previously demonstrated anorexigenic function of serotonin. Our results suggest that obesity-associated ETV5 has a food intake stimulating function and that this function is modulated through serotonin.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping