PUBLICATION
Comparative analyses of super-enhancers reveal conserved elements in vertebrate genomes
- Authors
- Pérez Rico, Y.A., Boeva, V., Mallory, A.C., Bitetti, A., Majello, S., Barillot, E., Shkumatava, A.
- ID
- ZDB-PUB-161215-3
- Date
- 2017
- Source
- Genome research 27(2): 259-268 (Journal)
- Registered Authors
- Bitetti, Angelo, Shkumatava, Alena
- Keywords
- none
- Datasets
- GEO:GSE75734
- MeSH Terms
-
- Acetylation
- Animals
- Chromatin/genetics*
- Conserved Sequence/genetics*
- Embryonic Development/genetics
- Enhancer Elements, Genetic*
- Gene Expression Regulation, Developmental
- Genomics
- Histones/genetics
- Humans
- Mice
- Transcription Factors/genetics
- Zebrafish/genetics*
- PubMed
- 27965291 Full text @ Genome Res.
Citation
Pérez Rico, Y.A., Boeva, V., Mallory, A.C., Bitetti, A., Majello, S., Barillot, E., Shkumatava, A. (2017) Comparative analyses of super-enhancers reveal conserved elements in vertebrate genomes. Genome research. 27(2):259-268.
Abstract
Super-enhancers (SEs) are key transcriptional drivers of cellular, developmental and disease states in mammals, yet the conservational and regulatory features of these enhancer elements in non-mammalian vertebrates are unknown. To define SEs in zebrafish and enable sequence and functional comparisons to mouse and human SEs, we used genome-wide histone H3 lysine 27 acetylation (H3K27ac) occupancy as a primary SE delineator. Our study determined the set of SEs in pluripotent state cells and adult zebrafish tissues and revealed both similarities and differences between zebrafish and mammalian SEs. Although the total number of SEs was proportional to the genome size, the genomic distribution of zebrafish SEs differed from that of the mammalian SEs. Despite the evolutionary distance separating zebrafish and mammals and the low overall SE sequence conservation, ~42% of zebrafish SEs were located in close proximity to orthologs that also were associated with SEs in mouse and human. Compared to their non-associated counterparts, higher sequence conservation was revealed for those SEs that have maintained orthologous gene associations. Functional dissection of two of these SEs identified conserved sequence elements and tissue-specific expression patterns, while chromatin accessibility analyses predicted transcription factors governing the function of pluripotent state zebrafish SEs. Our zebrafish annotations and comparative studies show the extent of SE usage and their conservation across vertebrates, permitting future gene regulatory studies in several tissues.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping